Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan, 611130, P. R. China.
Shandong Vocational Animal Science and Veterinary College, Weifang, Shandon, 261061, P. R. China.
Mol Nutr Food Res. 2018 Dec;62(24):e1800921. doi: 10.1002/mnfr.201800921. Epub 2018 Nov 9.
Daidzein, a natural isoflavone with estrogen-like activity, has been implicated in the regulation of reproductive performance in mammals. However, little is known about the molecular mechanisms involved. Here, the effects and potential mechanisms of daidzein supplementation on fetal growth in rats have been explored.
Thirty-six pregnant Sprague-Dawley rats are assigned to receive either an AIN-93M diet or an AIN-93M diet supplemented with 50 mg kg daidzein. Blood, placental, and fetus samples were collected on day 15 of gestation. It is shown that daidzein significantly improves the rat reproductive performance, which is associated with a higher fetus number, and the weight of the fetus and placenta (p < 0.05). Daidzein also increases the maternal serum estrogen and leptin concentrations, and the activity of superoxide dismutase (SOD) (p < 0.05). Notably, the isobaric tags for relative and absolute quantification (iTRAQ)-based proteomics analysis identifies 43 differentially expressed (DE) proteins in the placenta upon daidzein supplementation (p < 0.05). Interestingly, critical proteins involved in amino acid transport and metabolism, embryonic development, ubiquitination processes, and immune responses are upregulated in the daidzein group (p < 0.05).
These results not only indicate a beneficial effect of daidzein supplementation on reproductive performance but also offer potential mechanisms behind daidzein-facilitated fetal growth in rats.
大豆黄酮是一种具有雌激素样活性的天然异黄酮，已被认为参与了哺乳动物生殖性能的调节。然而，其涉及的分子机制知之甚少。本研究旨在探讨大豆黄酮补充对大鼠胎儿生长的影响及其潜在机制。
将 36 只怀孕的 Sprague-Dawley 大鼠分为两组，分别给予AIN-93M 饮食或 AIN-93M 饮食补充 50mg/kg 的大豆黄酮。在妊娠第 15 天收集血液、胎盘和胎儿样本。结果表明，大豆黄酮显著改善了大鼠的生殖性能，表现为胎儿数量增加，胎儿和胎盘的重量增加（p<0.05）。大豆黄酮还增加了母体血清雌激素和瘦素的浓度，以及超氧化物歧化酶（SOD）的活性（p<0.05）。值得注意的是，基于等重标记相对和绝对定量（iTRAQ）的蛋白质组学分析发现，大豆黄酮补充组胎盘中有 43 种差异表达蛋白（DE 蛋白）（p<0.05）。有趣的是，参与氨基酸转运和代谢、胚胎发育、泛素化过程和免疫反应的关键蛋白在大豆黄酮组中上调（p<0.05）。
这些结果不仅表明大豆黄酮补充对生殖性能有有益的影响，还为大豆黄酮促进大鼠胎儿生长的潜在机制提供了线索。
