Key Laboratory for Animal Disease Resistance Nutrition, Animal Nutrition Institute, Sichuan Agricultural University, Ya'an, People's Republic of China.
Br J Nutr. 2011 Aug;106(4):510-8. doi: 10.1017/S0007114511000614. Epub 2011 Apr 13.
The objective of the present study was to investigate the effects of the addition of fibre and the antioxidant N-acetylcysteine (NAC) to fat-rich diets on fetal intrauterine development in rats. A total of eighty virgin female Sprague-Dawley rats were fed a control diet, a high-fat diet (HF), a high-fat and high-fibre diet (HFF) or a high-fat NAC diet until day 19·5 of gestation. Maternal HFF consumption resulted in a significantly higher mean fetal number and placental weight than in the other groups (P < 0·05). The HFF diet significantly abrogated HF-induced decreases in maternal serum and placental superoxide anion and hydroxyl radical scavenging capacities (P < 0·05); partially abrogated HF-induced increases in maternal serum and placental malondialdehyde (MDA) and protein carbonyl concentrations (maternal serum MDA and placental protein carbonyl, P < 0·05); resulted in significantly higher fetal liver total superoxide dismutase (SOD), Cu- and Zn-containing SOD and Mn-containing SOD (Mn-SOD) activities than in the HF group (P < 0·05). Furthermore, mRNA expressions of hypoxia-inducible factor 1-α, thioredoxin 2 and Mn-SOD in fetal liver and Mn-SOD in fetal heart and placental GLUT3 in the HFF group were higher than those in the other groups (P < 0·05). The inclusion of dietary fibre in the HF diet was more effective than NAC supplementation in maintaining maternal serum and placental superoxide anion and hydroxyl radical scavenging capacities close to those of the control. These results suggest that maternal fibre intake during pregnancy is beneficial for fetal intrauterine development possibly through the improvement of maternal, placental and fetal antioxidant capacities and placental nutrient transfer capacity.
本研究旨在探讨在高脂肪饮食中添加纤维和抗氧化剂 N-乙酰半胱氨酸（NAC）对大鼠胎儿宫内发育的影响。总共 80 只雌性 Sprague-Dawley 大鼠分别喂食对照饮食、高脂肪饮食（HF）、高脂肪高纤维饮食（HFF）或高脂肪 NAC 饮食，直至妊娠第 19.5 天。与其他组相比，HFF 饮食组的平均胎儿数量和胎盘重量显著更高（P＜0.05）。HFF 饮食显著减弱了 HF 诱导的母体血清和胎盘中超氧阴离子和羟自由基清除能力的降低（P＜0.05）；部分减弱了 HF 诱导的母体血清和胎盘丙二醛（MDA）和蛋白质羰基浓度的增加（母体血清 MDA 和胎盘蛋白质羰基，P＜0.05）；导致胎儿肝脏总超氧化物歧化酶（SOD）、Cu 和 Zn 结合的 SOD 和 Mn 结合的 SOD（Mn-SOD）活性显著高于 HF 组（P＜0.05）。此外，HFF 组胎儿肝脏中缺氧诱导因子 1-α、硫氧还蛋白 2 和 Mn-SOD 的 mRNA 表达以及胎儿心脏和胎盘 GLUT3 的 Mn-SOD 表达均高于其他组（P＜0.05）。在 HF 饮食中添加膳食纤维比补充 NAC 更能有效维持母体血清和胎盘中超氧阴离子和羟自由基的清除能力接近对照水平。这些结果表明，母亲在怀孕期间摄入膳食纤维有利于胎儿宫内发育，可能是通过改善母体、胎盘和胎儿的抗氧化能力以及胎盘营养转运能力。
