Qiao Li-Na, Gao Qiao-Ling, Tan Lian-Hong, Shi Yi-Nan, Jiang Yan-Shu, Yang Jiao-Jiao, Yang Yong-Sheng
Lab of Biochemistry and Molecular Biology, Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, Beijing 100700, China.
Lab of Biochemistry and Molecular Biology, Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, Beijing 100700, China; College of Acupuncture-moxibustion and Massage, Changchun University of Traditional Chinese Medicine, Changchun 130117.
Zhen Ci Yan Jiu. 2018 Oct 25;43(10):651-6. doi: 10.13702/j.1000-0607.180317.
To observe the effect of electroacupuncture (EA) stimulation of single and multiple acupoints on sleep and concentrations of interlukin-1 β(IL-1 β), brain-derived neurotrophic factor (BDNF), prostaglandin D(PGD) and melatonin (MLT, sleep-promoting factors) and corticosterone (CORT, awakening-promoting factor) in the serum in insomnia rats, so as to explore its efficacy difference and the mechanism underlying improving sleep.
Fifty-four male SD rats were randomly divided into control, model, EA-Baihui (GV 20), EA-Shenmen (HT 7), EA-Sanyinjiao (SP 6) and EA-GV 20＋HT 7＋SP 6 groups (＝9 rats in each group). The insomnia model was established by intraperitoneal injection of para-chlorophenylalanine (PCPA, 300 mg/kg) once daily for 2 days. In the EA-GV 20, EA-HT 7, EA-SP 6 and EA-GV 20＋HT 7＋SP 6 groups, EA stimulation was administrated for 30 min, once a day for 4 days. The sleep onset latency and sleep duration were measured after intraperitoneal injection of pentobarbital sodium (35 mg/kg). The concentrations of IL-1 β， BDNF, MLT, PGDand CORT in the serum were detected by ELISA.
After EA stimulation of GV 20, HT 7, SP 6 and GV 20＋HT 7＋SP 6, the sleep latency was significantly shortened (<0.05, <0.01, except SP 6), and the sleep duration was remarkably prolonged in comparison with the model group (<0.05, <0.01), and the therapeutic effects of EA-GV 20＋HT 7＋SP 6 were significantly superior to those of EA-GV 20, EA-HT 7 and EA-SP 6 in shortening the sleep latency and lengthening the sleep duration (<0.05). Following modeling, the concentrations of IL-1 β， BDNF, PGD and MLT were significantly down-regulated, and the CORT level was markedly up-regulated in the model group relevant to the control group (<0.05). Following EA，modeling induced dramatic decrease of serum IL-1 β， BDNF, PGD and MLT was considerably up-regulated, and the increased CORT level markedly down-regulated in the EA-GV 20, EA-HT 7, EA-SP 6 and EA-GV 20＋HT 7＋SP 6 groups (<0.05). The effects of EA-GV 20＋HT 7＋SP 6 were evidently superior to those of EA-GV 20 and EA-SP 6 in up-regulating serum IL-1 β， BDNF and PGDlevels, and to those of HT 7, GV 20 and SP 6 in up-regulating serum MLT level, and significantly superior to those of EA-ST 7 and EA-SP 6 in down-regulating serum CORT (<0.05).
EA stimulation of HT 7, GV 20, SP 6 and GV 20＋HT 7＋ SP 6 can significantly improve the sleep in insomnia rats, which is closely associated with its effects in regulating serum sleep-promoting factors and awakening-promoting factor. Joint administration of EA of GV 20＋HT 7＋ SP 6 has a better effect than the single acupoint mentioned above.
观察电针（EA）刺激单穴与多穴对失眠大鼠睡眠及血清中白细胞介素-1β（IL-1β）、脑源性神经营养因子（BDNF）、前列腺素D（PGD）和褪黑素（MLT，促睡眠因子）以及皮质酮（CORT，促觉醒因子）浓度的影响，以探讨其疗效差异及改善睡眠的作用机制。
将54只雄性SD大鼠随机分为对照组、模型组、电针百会（GV 20）组、电针神门（HT 7）组、电针三阴交（SP 6）组和电针GV 20＋HT 7＋SP 6组（每组9只）。采用腹腔注射对氯苯丙氨酸（PCPA，300 mg/kg），每日1次，连续2天建立失眠模型。在电针GV 20组、电针HT 7组、电针SP 6组和电针GV 20＋HT 7＋SP 6组中，进行电针刺激30分钟，每天1次，共4天。腹腔注射戊巴比妥钠（35 mg/kg）后测量睡眠起始潜伏期和睡眠时间。采用酶联免疫吸附测定法（ELISA）检测血清中IL-1β、BDNF、MLT、PGD和CORT的浓度。
电针GV 20、HT 7、SP 6及GV 20＋HT 7＋SP 6后，与模型组比较，睡眠潜伏期显著缩短（除SP 6外，P<0.05，P<0.01），睡眠时间显著延长（P<0.05，P<0.01），且电针GV 20＋HT 7＋SP 6在缩短睡眠潜伏期和延长睡眠时间方面的治疗效果显著优于电针GV 20、电针HT 7和电针SP 6（P<0.05）。造模后，与对照组比较，模型组血清IL-1β、BDNF、PGD和MLT浓度显著下调，CORT水平显著上调（P<0.05）。电针后，电针GV 20组、电针HT 7组、电针SP 6组和电针GV 20＋HT 7＋SP 6组中，造模引起的血清IL-1β、BDNF、PGD显著降低及MLT显著升高均明显改善，升高的CORT水平显著下调（P<0.05）。电针GV 20＋HT 7＋SP 6在上调血清IL-1β、BDNF和PGD水平方面的作用明显优于电针GV 20和电针SP 6，在上调血清MLT水平方面优于电针HT 7、电针GV 20和电针SP 6，在下调血清CORT方面显著优于电针HT 7和电针SP 6（P<0.05）。
电针刺激HT 7、GV 20、SP 6及GV 20＋HT 7＋SP 6可显著改善失眠大鼠的睡眠，这与其调节血清促睡眠因子和促觉醒因子密切相关。电针GV 20＋HT 7＋SP 6联合应用的效果优于上述单穴。
