Chandra Jyotsna, Retuerto Mauricio, Seité Sophie, Martin Richard, Kus Michaela, Ghannoum Mahmoud A, Baron Elma, Mukherjee Pranab K
J Drugs Dermatol. 2018 Oct 1;17(10):1039-1048.
Atopic dermatitis (AD) is associated with changes in skin bacterial microbiome. Emollient treatment induces change in bacterial microbiome in AD, but its effect on fungal microbiome ("mycobiome") and their inter-kingdom correlations is unknown. We used Ion-Torrent sequencing to characterize the mycobiome of AD patients in response to emollient treatment.
Skin swabs were collected from lesional and non-lesional skin of AD patients suffering from moderate AD, after informed consent and according to GCP guidelines. Genomic DNA was extracted from each swab using the MoBio PowerSoil DNA Isolation kit and used for mycobiome sequencing analyses as described in our earlier publications. Principal coordinates analyses (PCoA), diversity, abundance, and correlations analyses were conducted in R and relevant packages using non-parametric tests (P less than .05 was significant).
Swab samples from 10 patients (7 females, 3 males; mean age, 10.5 years) were analyzed. Emollient treatment induced a significant reduction of Scoring Atopic Dermatitis (SCORAD) score (P less than .001). PCoA showed pre-treatment and post-treatment samples clustered differently at all taxa levels. Six genera were detected in only non-lesional samples, while four were detected in only lesional samples. In non-lesional samples, Shannon diversity index was significantly increased after emollient treatment (P less than equal to .04), while lesional skin exhibited non-significant decrease. Ascomycota was the most abundant phylum and Dothideomycetes was the most abundant Class in most samples. Eight fungal species were either significantly different (P less than .05) or showed a strong trend (P less than .1) between pre- and post-treatment samples of lesional and non-lesional skin. In lesional skin, Gram-negative Pseudomonas spp. correlated significantly with pathogenic fungal species (Aspergillus, Candida spp.) in pre-treatment samples; these correlations were not detected in post-treatment samples. Moreover, lesional skin exhibited significant correlations between Gram-positive bacteria (Corynebacterium kroppenstedtiian and Staphylococcus pettenkoferi) and pathogenic Candida species in pre-treatment samples, but not in post- treated samples.
Emollient treatment may induce beneficial microbial changes in the mycobiome and augment host-microbe balance on skin in AD. Clinical relevance of these results need to be investigated. J Drugs Dermatol. 2018;17(10):1039-1048.
特应性皮炎（AD）与皮肤细菌微生物群的变化有关。润肤剂治疗可诱导AD患者细菌微生物群的变化，但其对真菌微生物群（“真菌群落”）及其跨王国相关性的影响尚不清楚。我们使用离子激流测序来表征AD患者在接受润肤剂治疗后的真菌群落。
在获得知情同意后，根据GCP指南，从患有中度AD的患者的皮损和非皮损皮肤中采集皮肤拭子。使用MoBio PowerSoil DNA分离试剂盒从每个拭子中提取基因组DNA，并按照我们早期出版物中所述用于真菌群落测序分析。在R和相关软件包中使用非参数检验进行主坐标分析（PCoA）、多样性、丰度和相关性分析（P小于0.05具有显著性）。
分析了10名患者（7名女性，3名男性；平均年龄10.5岁）的拭子样本。润肤剂治疗导致特应性皮炎评分（SCORAD）显著降低（P小于0.001）。PCoA显示，在所有分类水平上，治疗前和治疗后的样本聚类不同。仅在非皮损样本中检测到6个属，而仅在皮损样本中检测到4个属。在非皮损样本中，润肤剂治疗后香农多样性指数显著增加（P小于等于0.04），而皮损皮肤则呈现不显著的下降。在大多数样本中，子囊菌门是最丰富的门，座囊菌纲是最丰富的纲。在皮损和非皮损皮肤的治疗前和治疗后样本之间，8种真菌物种存在显著差异（P小于0.05）或呈现强烈趋势（P小于0.1）。在皮损皮肤中，革兰氏阴性假单胞菌属在治疗前样本中与致病真菌物种（曲霉属、念珠菌属）显著相关；在治疗后样本中未检测到这些相关性。此外，在治疗前样本中，皮损皮肤在革兰氏阳性菌（克氏棒状杆菌和佩氏葡萄球菌）与致病念珠菌物种之间呈现显著相关性，但在治疗后样本中未呈现。
润肤剂治疗可能诱导真菌群落中有益的微生物变化，并增强AD患者皮肤的宿主-微生物平衡。这些结果的临床相关性需要进一步研究。《药物皮肤病学杂志》。2018年；17（10）：1039-1048。
