Maehara Y, Anai H, Kusumoto H, Sugimachi K
Eur J Surg Oncol. 1987 Jun;13(3):203-6.
The chemosensitivities of 41 poorly differentiated gastric cancer tissues were compared with that of 16 well differentiated tissues, using the in vitro succinate dehydrogenase inhibition test. These human tissues obtained at the time of surgery were exposed to six different antitumor drugs: carboquone (CQ), adriamycin (ADM), mitomycin C (MMC), aclacinomycin A (ACR), cisplatin (DDP) and 5-fluorouracil (5-FU). The chemosensitivity was determined as positive when the succinate dehydrogenase (SD) activity of the drug exposed cells was decreased to below 50% of that of control cells, on day 3 of exposure. Decrease in SD activity was remarkable in the poorly differentiated tissues, compared to the well differentiated tissues, exposed to ADM, MMC, DDP and 5-FU. The sensitive rates were higher in the poorly differentiated tissues than in the well differentiated tissues, against all six antitumor drugs. Sixty-three per cent of the poorly differentiated tissues were sensitive to more than three antitumor drugs, in an identical tissue, but the rate was only 19% in the well differentiated tissues. The resistant rates to all drugs tested were 20% in the poorly differentiated and 31% in the well differentiated tissues. This would indicate that patients with a poorly differentiated gastric cancer will probably show a better response to antitumor drugs, compared to those with a well differentiated type.
采用体外琥珀酸脱氢酶抑制试验，比较了41例低分化胃癌组织与16例高分化组织的化学敏感性。这些手术时获取的人体组织被暴露于六种不同的抗肿瘤药物：卡波醌（CQ）、阿霉素（ADM）、丝裂霉素C（MMC）、阿克拉霉素A（ACR）、顺铂（DDP）和5-氟尿嘧啶（5-FU）。当暴露于药物的细胞的琥珀酸脱氢酶（SD）活性在暴露第3天时降至对照细胞活性的50%以下时，化学敏感性被判定为阳性。与高分化组织相比，暴露于ADM、MMC、DDP和5-FU的低分化组织中SD活性的降低更为显著。在所有六种抗肿瘤药物方面，低分化组织的敏感率高于高分化组织。63%的低分化组织对同一组织中的三种以上抗肿瘤药物敏感，但高分化组织中的这一比例仅为19%。低分化组织对所有测试药物的耐药率为20%，高分化组织为31%。这表明，与高分化型胃癌患者相比，低分化胃癌患者可能对抗肿瘤药物表现出更好的反应。
