College of Marine Science and Engineering, Nanjing Normal University, Nanjing, Jiangsu 210023, China; Co-Innovation Center for Marine Bio-Industry Technology of Jiangsu Province, Lianyungang, Jiangsu 222005, China.
College of Marine Science and Engineering, Nanjing Normal University, Nanjing, Jiangsu 210023, China.
Fish Shellfish Immunol. 2019 Jan;84:648-655. doi: 10.1016/j.fsi.2018.10.053. Epub 2018 Oct 23.
Copper nanoparticles (Cu NPs) are a new pollutant in aquaculture, representing a hazard to aquatic organisms. We investigated the effects of Cu NPs exposure on oxidative stress, apoptosis and immune response in an economically important model species, Takifugu fasciatus. The juvenile fish were exposed to control, 20 or 100 μg Cu NPs/L for 30 days. The growth of T. fasciatus was inhibited after Cu NPs exposure. Copper accumulation in liver increased with increasing Cu NPs dose. Oxidative stress indicators [malondialdehyde (MDA), total superoxide dismutase (T-SOD), catalase (CAT) and glutathione (GSH)], apoptosis index and activities of caspases (caspase-3, caspase-9) were all increased with the increase of Cu NPs concentration in liver. With an increase in Cu NPs dose, the activities of succinate dehydrogenase (SDH) and Na-K-ATPase as well as cytochrome c (Cyt-c) concentration in mitochondria decreased, accompanied by increased Cyt-c concentration in cytosol. Apoptosis-related gene expressions of p53, caspase-3, caspase-9 and Bax were increased with the increase of Cu NPs dose. However, the opposite result was found in Bcl2 expression. The physiological indicators of immune response [heat shock protein 70 (HSP70), heat shock protein 90 (HSP90), immunoglobulin M (IgM) and lysozyme (LZM)] as well as the mRNA levels of HSP70, HSP90, IgM and C-LZM were all increased after Cu NPs exposure. Our results will be helpful in understanding the mechanism of Cu NPs toxicity in T. fasciatus.
铜纳米粒子（Cu NPs）是水产养殖中的一种新型污染物，对水生生物构成危害。我们研究了 Cu NPs 暴露对一种经济重要模式物种——红鳍东方鲀（Takifugu fasciatus）的氧化应激、细胞凋亡和免疫反应的影响。幼鱼暴露于对照、20 或 100μg Cu NPs/L 中 30 天。Cu NPs 暴露后，红鳍东方鲀的生长受到抑制。肝脏中铜的积累随 Cu NPs 剂量的增加而增加。随着肝脏中 Cu NPs 浓度的增加，氧化应激指标[丙二醛（MDA）、总超氧化物歧化酶（T-SOD）、过氧化氢酶（CAT）和谷胱甘肽（GSH）]、凋亡指数和半胱天冬酶（caspase-3、caspase-9）的活性均升高。随着 Cu NPs 剂量的增加，线粒体中琥珀酸脱氢酶（SDH）和 Na-K-ATP 酶的活性以及细胞色素 c（Cyt-c）浓度降低，同时细胞质中 Cyt-c 浓度增加。凋亡相关基因 p53、caspase-3、caspase-9 和 Bax 的表达随 Cu NPs 剂量的增加而增加。然而，Bcl2 的表达却出现相反的结果。免疫反应的生理指标[热休克蛋白 70（HSP70）、热休克蛋白 90（HSP90）、免疫球蛋白 M（IgM）和溶菌酶（LZM）]以及 HSP70、HSP90、IgM 和 C-LZM 的 mRNA 水平在 Cu NPs 暴露后均升高。我们的研究结果将有助于理解 Cu NPs 对红鳍东方鲀毒性的机制。
