Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Stem Cell Technology Research Center, Tehran, Iran.
J Pharm Biomed Anal. 2019 Feb 5;164:104-111. doi: 10.1016/j.jpba.2018.09.048. Epub 2018 Oct 10.
In this study, graphene oxide (GO) and reduced graphene oxide (rGO) were used as visual labels in a lateral flow assay for detection of E. coli O157:H7. The color intensity was employed for the quantitative measurements of the target bacteria. Quantitative results showed that in comparison to GO, rGO can provide higher color intensity owing to enhanced light absorption following chemical reduction. Our results confirm that the visual limit of detection of the target bacteria by rGO is ∼10 colony forming unit per milliliter (CFU/ml), which closely compares with current alternative techniques using gold nanoparticles. The performance and practicability of the rGO-based test strips for detection of the target bacteria in milk and drinking water were validated with conventional plating and colony counting techniques. Results suggest that the proposed lateral flow assay is sensitive, specific, and affordable. It has also the potential to become a widely used detection technique for E. coli O157:H7 and a wide variety of other analytes.
在这项研究中，氧化石墨烯（GO）和还原氧化石墨烯（rGO）被用作侧向流动分析中的可视化标记物，用于检测大肠杆菌 O157:H7。颜色强度用于对目标细菌进行定量测量。定量结果表明，与 GO 相比，rGO 可以提供更高的颜色强度，因为化学还原后增强了光吸收。我们的结果证实，rGO 对目标细菌的可视化检测限约为 10 个每毫升菌落形成单位（CFU/ml），与当前使用金纳米粒子的替代技术相当。使用传统的平板和菌落计数技术验证了基于 rGO 的测试条在牛奶和饮用水中检测目标细菌的性能和实用性。结果表明，所提出的侧向流动分析具有灵敏、特异和经济的特点。它还有可能成为一种广泛应用的大肠杆菌 O157:H7 检测技术以及各种其他分析物的检测技术。
