College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China.
College of Food Science and Engineering, Qingdao Agricultural University, Qingdao 266109, China.
Int J Mol Sci. 2018 Oct 25;19(11):3323. doi: 10.3390/ijms19113323.
To optimize the physicochemical properties of phthalocyanine (PC), we examined its behavior in particles of triple helix glucan curdlan (CUR). CUR was denatured and renatured in DMSO, in the presence of PC. Infrared spectroscopy and transmission electron microscopy (TEM) showed that PC and CUR formed an inclusion complex, in which PC was trapped inside CUR molecules. This redshifted the absorption peak of PC, which would improve its usefulness as a photosensitizer, because infrared light can penetrate more deeply into human tissues. The conductivity of the solution of CUR-PC was higher than the conductivities of either a CUR solution or a PC dispersion, indicating that CUR-PC is more water soluble than PC. In addition, CUR-PC was highly stable in water. Thus, the use of CUR as a carrier of PC improves several of its physical properties. PC is used as a photosensitizer for killing cancer cells, but its use is hampered by its low solubility. Further, its absorption range limits its use to a depth of 1⁻3 mm in tissues. CUR-PC, with its high solubility and infrared absorption peak, was highly effective as a photosensitizer. It killed 84% of HeLa cells under 15 min of long wavelength radiation and had little cytotoxicity in the absence of light. These results demonstrate that CUR-PC has promise as a photosensitizer, as well as provide theoretical support for a wide range of applications for PC and CUR.
为了优化酞菁（PC）的物理化学性质，我们研究了它在三螺旋葡聚糖（CUR）颗粒中的行为。在 DMSO 中，PC 存在的情况下，CUR 变性和复性。红外光谱和透射电子显微镜（TEM）表明，PC 和 CUR 形成了一种包合物，其中 PC 被困在 CUR 分子内。这使 PC 的吸收峰红移，这将提高其作为光增敏剂的有用性，因为红外光可以更深入地穿透人体组织。CUR-PC 溶液的电导率高于 CUR 溶液或 PC 分散体的电导率，表明 CUR-PC 比 PC 更易溶于水。此外，CUR-PC 在水中高度稳定。因此，CUR 作为 PC 的载体可以改善其几种物理性质。PC 用作杀死癌细胞的光增敏剂，但由于其溶解度低，其使用受到限制。此外，其吸收范围限制了其在组织中的使用深度为 1⁻3mm。具有高溶解度和红外吸收峰的 CUR-PC 作为光增敏剂非常有效。在 15 分钟的长波长辐射下，它杀死了 84%的 HeLa 细胞，在没有光照的情况下几乎没有细胞毒性。这些结果表明 CUR-PC 作为光增敏剂具有很大的潜力，并为 PC 和 CUR 的广泛应用提供了理论支持。
