Malaysian Palm Oil Board, No. 6, Persiaran Institusi, Bandar Baru Bangi, 43000, Kajang, Selangor, Malaysia.
Protein J. 2018 Dec;37(6):473-499. doi: 10.1007/s10930-018-9802-x.
Proteomics technologies were first applied in the oil palm research back in 2008. Since proteins are the gene products that are directly correspond to phenotypic traits, proteomic tools hold a strong advantage above other molecular tools to comprehend the biological and molecular mechanisms in the oil palm system. These emerging technologies have been used as non-overlapping tools to link genome-wide transcriptomics and metabolomics-based studies to enhance the oil palm yield and quality through sustainable plant breeding. Many efforts have also been made using the proteomics technologies to address the oil palm's Ganoderma disease; the cause and management. At present, the high-throughput screening technologies are being applied to identify potential biomarkers involved in metabolism and cellular development through determination of protein expression changes that correlate with oil production and disease. This review highlights key elements in proteomics pipeline, challenges and some examples of their implementations in plant studies in the context of oil palm in particular. We foresee that the proteomics technologies will play more significant role to address diverse issues related to the oil palm in the effort to improve the oil crop.
蛋白质组学技术早在 2008 年就首次应用于油棕研究。由于蛋白质是直接对应表型特征的基因产物，因此蛋白质组学工具在理解油棕系统中的生物学和分子机制方面比其他分子工具具有更强的优势。这些新兴技术已被用作非重叠的工具，将全基因组转录组学和基于代谢组学的研究联系起来，通过可持续的植物育种来提高油棕的产量和质量。许多研究还利用蛋白质组学技术来解决油棕的灵芝病问题，包括病因和管理。目前，高通量筛选技术正在应用于通过确定与产油和疾病相关的蛋白质表达变化来鉴定与代谢和细胞发育相关的潜在生物标志物。本文重点介绍了蛋白质组学研究中的关键环节、挑战以及它们在油棕植物研究中的一些应用实例。我们预计蛋白质组学技术将在解决与油棕相关的各种问题方面发挥更重要的作用，以提高油作物的产量。
