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测量单根大鼠骨骼肌纤维中的葡萄糖摄取和肌球蛋白重链同工型表达

Measuring Both Glucose Uptake and Myosin Heavy Chain Isoform Expression in Single Rat Skeletal Muscle Fibers.

作者信息

Pataky Mark W, Arias Edward B, Cartee Gregory D

机构信息

Muscle Biology Laboratory, School of Kinesiology, University of Michigan, Ann Arbor, MI, USA.

Department of Molecular and Integrative Physiology, University of Michigan, Ann Arbor, MI, USA.

出版信息

Methods Mol Biol. 2019;1889:283-300. doi: 10.1007/978-1-4939-8897-6_17.

Abstract

Glucose uptake by skeletal muscle is important for metabolic health. Because skeletal muscle is composed of multiple fiber types that have differing metabolic and contractile properties, studying glucose uptake in whole muscle tissue does not elucidate differences at the cellular level. Here, we describe a procedure that enables the measurement of both glucose uptake and fiber type (by myosin heavy chain isoform expression) in individual rat epitrochlearis muscle fibers.

摘要

骨骼肌对葡萄糖的摄取对代谢健康很重要。由于骨骼肌由多种具有不同代谢和收缩特性的纤维类型组成,因此在整个肌肉组织中研究葡萄糖摄取并不能阐明细胞水平上的差异。在这里,我们描述了一种方法,该方法能够测量单个大鼠肱三头肌肌纤维中的葡萄糖摄取和纤维类型(通过肌球蛋白重链同工型表达)。

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