Oestbye Tone-Kari K, Ytteborg Elisabeth
Nofima AS, Ås, Norway.
Methods Mol Biol. 2019;1889:319-330. doi: 10.1007/978-1-4939-8897-6_19.
This chapter outlines methods for isolating myosatellites from Atlantic salmon (Salmo salar), how to keep them in culture and differentiate them into mature myocytes. The protocol further describes how to trans-differentiate the myocytes into osteoblasts (bone cells).
本章概述了从大西洋鲑鱼(Salmo salar)中分离肌卫星细胞的方法,以及如何在培养中保存它们并将其分化为成熟的肌细胞。该方案还进一步描述了如何将肌细胞转分化为成骨细胞(骨细胞)。