Matschak T W, Stickland N C
Royal Veterinary College, London, England, UK.
Experientia. 1995 Mar 15;51(3):260-6. doi: 10.1007/BF01931109.
Temperature is known to affect fish growth, and in Atlantic salmon there is an influence on muscle cellularity. Primary muscle cell culture makes it possible to investigate direct effects of temperature on myogenic cells. Salmon myosatellite cells were cultured for the first time in this study. The cells were cultured at either 5 degrees C or 11 degrees C. Increased temperature led to an increase in differentiation rate and especially hypertrophic growth (Q10 = 4.0). No nuclear proliferation was evident in the satellite cell population isolated at either temperature. This may be due to the presence of different subpopulations of myogenic cells at different developmental ages or the presence of indirect factors in vivo.
已知温度会影响鱼类生长，对于大西洋鲑鱼而言，温度会对肌肉细胞构成产生影响。原代肌肉细胞培养使得研究温度对成肌细胞的直接影响成为可能。在本研究中首次对鲑鱼肌卫星细胞进行了培养。细胞分别在5摄氏度或11摄氏度下培养。温度升高导致分化率增加，尤其是肥大生长（Q10 = 4.0）。在任一温度下分离出的卫星细胞群体中均未观察到明显的核增殖。这可能是由于存在不同发育阶段的成肌细胞亚群，或者体内存在间接因素。
