Key Laboratory of Neuroregeneration, Neural Regeneration Co-Innovation Center of Jiangsu Province, Nantong University, Nantong, 226001, People's Republic of China.
Department of Chemistry, Brandeis University, 415 South Street, Waltham, Massachusetts, 02454.
J Biomed Mater Res A. 2019 Jan;107(1):104-113. doi: 10.1002/jbm.a.36537. Epub 2018 Oct 26.
In tissue regenerative medicine, developing tunable degradation rate of biomaterials for predictive functional outcomes remains critical. The implanted scaffolds should degrade gradually along with the tissue regeneration, and the optimal degradation rate of scaffold depends on the tissue type to be regenerated. Herein, the tunable degradation rates of silk fibroin (SF) scaffolds were fabricated through controlling dissolution, hydrolyzing conditions, and freeze-drying. The pore size, water adsorption capacity, and mechanical properties of scaffolds were associated with their average molecular weights. Moreover, in vitro cytotoxicity tests demonstrated that rapid degradation of SF scaffolds would facilitate the Schwann cells proliferation. Furthermore, in vitro enzymatic degradation and in vivo subcutaneous implantation experiments illustrated that SF scaffolds degradation behaviors could be well regulated. Immunohistochemistry staining experiments suggested that SF scaffold-degradation products could promote the endothelial cells proliferation. These results indicate that SF tunable degradation rates are promising candidates in regenerative medicine. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 104-113, 2019.
在组织再生医学中，开发可预测功能结果的生物材料可调降解率仍然至关重要。植入的支架应随着组织再生而逐渐降解，而支架的最佳降解率取决于要再生的组织类型。在此，通过控制溶解、水解条件和冷冻干燥来制备丝素（SF）支架的可调降解率。支架的孔径、吸水率和机械性能与其平均分子量有关。此外，体外细胞毒性试验表明 SF 支架的快速降解有利于施万细胞的增殖。此外，体外酶降解和体内皮下植入实验表明 SF 支架的降解行为可以得到很好的调节。免疫组织化学染色实验表明 SF 支架降解产物可以促进内皮细胞的增殖。这些结果表明 SF 的可调降解率是再生医学中有前途的候选材料。© 2018 年威利父子公司。J 生物医学材料研究部分 A：107A：104-113，2019 年。
