Yamada M, Saier M H
J Bacteriol. 1987 Jul;169(7):2990-4. doi: 10.1128/jb.169.7.2990-2994.1987.
The glucitol (gut) operon has been identified in the colony bank of Clark and Carbon (A. Sancar and W. D. Rupp, Proc. Natl. Acad. Sci. USA 76:3144-3148, 1979). We subcloned the gut operon by using paCYC184, pACYC177, and pBR322. The operon, which is encoded in a 3.3-kilobase nucleotide fragment, consists of the gutC, gutA, gutB, and gutD genes. The repressor of the gut operon seemed to be encoded in the region downstream from the operon. The gene products of the gut operon were identified by using maxicells. The apparent molecular weights of the glucitol-specific enzyme II (product of the gutA gene), enzyme III (product of the gutB gene), and glucitol-6-phosphate dehydrogenase (product of the gutD gene) were about 46,000, 13,500, and 27,000, respectively, as estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
在Clark和Carbon的菌落文库中已鉴定出葡萄糖醇（gut）操纵子（A. Sancar和W. D. Rupp，《美国国家科学院院刊》76:3144 - 3148，1979年）。我们使用pACYCl84、pACYC177和pBR322对gut操纵子进行了亚克隆。该操纵子由一个3.3千碱基的核苷酸片段编码，包含gutC、gutA、gutB和gutD基因。gut操纵子的阻遏物似乎在操纵子下游区域编码。通过使用最大细胞对gut操纵子的基因产物进行了鉴定。经十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳估计，葡萄糖醇特异性酶II（gutA基因产物）、酶III（gutB基因产物）和葡萄糖醇 - 6 - 磷酸脱氢酶（gutD基因产物）的表观分子量分别约为46,000、13,500和27,000。
