Sudan Vikrant, Shanker Daya, Jaiswal Amit Kumar
Department of Veterinary Parasitology, College of Veterinary Sciences and Animal Husbandry, U.P. Pandit Deen Dayal Upadhyaya Pashu Chikitsa VigyanVishwavidyalaya Evam Go Anusandhan Sansthan (DUVASU), Mathura-281001, India.
Acta Parasitol. 2018 Dec 19;63(4):781-783. doi: 10.1515/ap-2018-0093.
Linguatula serrata is a pentastomid which is worldwide in distribution. However, a very few references are reported from India. In the present study, the cox I gene of L. serrata nymphs, originally isolated from mesenteric lymph nodes of buffaloes, was amplified and custom sequenced. Based on sequence analysis, two haplotypes were noticed and were subsequently submitted in NCBI database. The sequences were also compared with the other sequences available in the pubmed and phylogenetic analysis coupled with nucleotide homologies were commutated. The studied Indian isolates were found closer to Bangladesh and Iran isolates. This is the first report of molecular characterization of L. serrata from India.
锯齿舌形虫是一种分布于全球的五口吸虫。然而，印度仅有极少数相关报道。在本研究中，从水牛肠系膜淋巴结中分离出的锯齿舌形虫若虫的细胞色素氧化酶亚基I（cox I）基因被扩增并进行了定制测序。基于序列分析，发现了两种单倍型，并随后提交至NCBI数据库。这些序列还与pubmed中可用的其他序列进行了比较，并进行了系统发育分析以及核苷酸同源性计算。研究发现，印度分离株与孟加拉国和伊朗的分离株更为接近。这是印度关于锯齿舌形虫分子特征的首次报道。
