Ghorashi Seyed Ali, Tavassoli Mousa, Peters Andrew, Shamsi Shokoofeh, Hajipour Naser
Acta Parasitol. 2016 Jan;61(1):195-200.
The phylogenetic relationships among seven Linguatula serrata (L. serrata) isolates collected from cattle, goats, sheep, dogs and camels in different geographical locations of Iran were investigated using partial 18S ribosomal RNA (rRNA) and partial mitochondrial cytochrome c oxidase subunit 1 (cox1) gene sequences. The nucleotide sequences were analysed in order to determine the phylogenetic relationships between the isolates. Higher sequence diversity and intraspecies variation was observed in the cox1 gene compared to 18S rRNA sequences. Phylogenetic analysis of the cox1 gene placed all L. serrata isolates in a sister clade to L. arctica. The Mantel regression analysis revealed no association between genetic variations and host species or geographical location, perhaps due to the small sample size. However, genetic variations between L. serrata isolates in Iran and those isolated in other parts of the world may exist and could reveal possible evolutionary relationships.
利用部分18S核糖体RNA（rRNA）和部分线粒体细胞色素c氧化酶亚基1（cox1）基因序列，对从伊朗不同地理位置的牛、山羊、绵羊、狗和骆驼身上采集的7株锯齿舌形虫（L. serrata）分离株之间的系统发育关系进行了研究。分析核苷酸序列以确定分离株之间的系统发育关系。与18S rRNA序列相比，cox1基因中观察到更高的序列多样性和种内变异。cox1基因的系统发育分析将所有锯齿舌形虫分离株置于与北极舌形虫（L. arctica）的姐妹进化枝中。Mantel回归分析显示遗传变异与宿主物种或地理位置之间没有关联，这可能是由于样本量较小。然而，伊朗的锯齿舌形虫分离株与世界其他地区分离的菌株之间可能存在遗传变异，并可能揭示可能的进化关系。
