Au D C, Gennis R B
J Bacteriol. 1987 Jul;169(7):3237-42. doi: 10.1128/jb.169.7.3237-3242.1987.
The structural genes encoding the cytochrome o terminal oxidase complex (cyo) of Escherichia coli have been subcloned into the multicopy plasmid pBR322 after the Mu-mediated transposition of the gene locus from the bacterial chromosome onto the conjugative R plasmid RP4. Introduction of cyo plasmids into strains (cyo cyd) lacking both terminal oxidases restored the ability of the strains to grow aerobically on nonfermentable substrates. Strains carrying the cyo plasmids produced 5 to 10 times more cytochrome o oxidase than did control strains. The gene products encoded by the cyo plasmids could be immunoprecipitated with monospecific antibodies raised against cytochrome o. The cloned genes will be valuable for studying the structure, function, and regulation of the cytochrome o terminal oxidase complex.
在通过Mu介导将编码大肠杆菌细胞色素o末端氧化酶复合物（cyo）的基因座从细菌染色体转座到接合性R质粒RP4上之后，这些结构基因已被亚克隆到多拷贝质粒pBR322中。将cyo质粒导入缺乏两种末端氧化酶的菌株（cyo cyd）后，恢复了这些菌株在非发酵底物上需氧生长的能力。携带cyo质粒的菌株产生的细胞色素o氧化酶比对照菌株多5至10倍。cyo质粒编码的基因产物可用针对细胞色素o产生的单特异性抗体进行免疫沉淀。这些克隆的基因对于研究细胞色素o末端氧化酶复合物的结构、功能和调控将具有重要价值。
