Pathogen Molecular Biology Department, Faculty of Infectious and Tropical Diseases, London School of Hygiene & Tropical Medicine, Keppel Street, London, UK.
Pathogen Molecular Biology Department, Faculty of Infectious and Tropical Diseases, London School of Hygiene & Tropical Medicine, Keppel Street, London, UK.
Int J Parasitol. 2018 Dec;48(14):1073-1078. doi: 10.1016/j.ijpara.2018.09.002. Epub 2018 Oct 24.
Malaria parasite oocysts located on the mosquito midgut generate sporozoites by a process called sporogony. Plasmodium berghei parasites express six LCCL lectin domain adhesive-like proteins (LAPs), which operate as a complex and share a localisation in the crystalloid - an organelle found in the ookinete and young oocyst. Depletion of LAPs prevents crystalloid formation, increases oocyst growth, and blocks sporogony. Here, we describe a LAP4 mutant that has abnormal crystalloid biogenesis and produces oocysts that display reduced growth and premature sporogony. These findings provide evidence for a role of the LAP complex in regulating oocyst cell division via the crystalloid.
疟原虫卵囊位于蚊子的中肠内，通过一种叫做孢子发生的过程产生孢子。伯氏疟原虫寄生虫表达六个 LCCL 凝集素结构域粘附样蛋白 (LAPs)，它们作为一个复合物发挥作用，并在晶体 - 一种在动合子和早期卵囊中发现的细胞器中具有局部定位。LAPs 的耗竭阻止了晶体的形成，增加了卵囊的生长，并阻断了孢子发生。在这里，我们描述了一个 LAP4 突变体，它具有异常的晶体发生，并产生显示出生长减少和过早孢子发生的卵囊。这些发现为 LAP 复合物通过晶体调节卵囊细胞分裂提供了证据。
