Fondazione Bruno Kessler, Center for Materials and Microsystems, Lab. Biomarker Studies and Structure Analysis for Health, via Sommarive 18, I-38123 Povo (Trento), Italy; CNR - Consiglio Nazionale delle Ricerche, Istituto di Biofisica, via alla Cascata 56/C, I-38123, Povo (Trento), Italy.
Fondazione Bruno Kessler, Center for Materials and Microsystems, Lab. Biomarker Studies and Structure Analysis for Health, via Sommarive 18, I-38123 Povo (Trento), Italy; CNR - Consiglio Nazionale delle Ricerche, Istituto di Biofisica, via alla Cascata 56/C, I-38123, Povo (Trento), Italy.
Talanta. 2019 Feb 1;193:44-50. doi: 10.1016/j.talanta.2018.09.080. Epub 2018 Sep 25.
Polydimethylsiloxane (PDMS) is a well-known biocompatible polymer employed for many applications in the biomedical field. In this study, the biocompatibility and versatility of PDMS was tested setting up a microdevice devoted to the purification and analysis of nucleic acids. The PDMS microdevice was demonstrated to successfully fulfill all requirements of genetic analyses such as genotyping and pathogen DNA identification both in multiplex and real-time PCR, suggesting the possibility to carry out a molecular test directly on-chip. Moreover, the PDMS microdevice was successfully applied to the purification and detection of disease biomarkers, such as microRNAs related to cancer or heart disease. On-chip microRNA purification was demonstrated starting from clinically relevant samples, i.e. plasma, serum, tissue biopsies. Significantly, the purification and the transcription of microRNA into cDNA occur in the same PDMS chamber, saving time and labor for the overall analysis. Again, the PDMS microdevice was confirmed as a notable candidate for compact, rapid, easy-to-use molecular tests.
聚二甲基硅氧烷（PDMS）是一种众所周知的生物相容性聚合物，广泛应用于生物医学领域的多种应用。在这项研究中，测试了 PDMS 的生物相容性和多功能性，建立了一个微设备，专门用于核酸的纯化和分析。PDMS 微设备成功地满足了基因分析的所有要求，如多重和实时 PCR 中的基因分型和病原体 DNA 鉴定，表明有可能直接在芯片上进行分子测试。此外，PDMS 微设备还成功地应用于疾病生物标志物的纯化和检测，如与癌症或心脏病相关的 microRNAs。从临床相关样本（即血浆、血清、组织活检）中证明了微 RNA 的芯片上纯化和检测。重要的是，微 RNA 的纯化和转录成 cDNA 都发生在同一个 PDMS 腔室内，为整体分析节省了时间和劳动力。同样，PDMS 微设备被确认为一种用于紧凑、快速、易于使用的分子测试的优秀候选者。
