Maurye Praveen, Basu Arpita, Sen Sohini, Biswas Jayanta Kumar, Bandyopadhyay Tapas Kumar, Naskar Malay
ICAR-Central Inland Fisheries Research Institute, Barrackpore, Kolkata, West Bengal, India.
School of Bioscience and Engineering, Jadavpur University, Kolkata, West Bengal, India.
Biochem Mol Biol Educ. 2018 Sep;46(5):566-577. doi: 10.1002/bmb.21172.
Recent advancements in biochemical sciences have helped the researchers to explore the molecular logic of life inclusive of its multifarious expressions and explain many facts about the structure and functions of cellular macromolecules. Due to its simple and cost-effective nature, polyacrylamide gel electrophoresis (PAGE) has become the most favored technique for qualitative and quantitative examination of macromolecules. Major drawbacks of such modifications are the cost and operational complexities faced by naïve students. Many interlinking laboratory equipment are needed in the school laboratories for the conduct of even simple scientific experiment. Some of these costly modern equipment are inaccessible for students of small laboratories, and their alternatives are not easily available. Many of these laboratory equipment required for routine gel electrophoresis technique can be fabricated in their simplest form using off-the-shelf components. A short term biochemistry training program was executed for high school students to provide them "hands-on" training using newly modified equipment, which was proved to be an exciting way of learning biochemical gel separation techniques. © 2018 International Union of Biochemistry and Molecular Biology, 46(5):566-577, 2018.
生物化学科学的最新进展帮助研究人员探索了生命的分子逻辑，包括其多种多样的表现形式，并解释了许多关于细胞大分子结构和功能的事实。由于聚丙烯酰胺凝胶电泳（PAGE）简单且成本效益高，它已成为大分子定性和定量检测最受欢迎的技术。这种改进的主要缺点是初学者面临的成本和操作复杂性。即使是进行简单的科学实验，学校实验室也需要许多相互关联的实验室设备。小型实验室的学生无法使用其中一些昂贵的现代设备，而且它们的替代品也不容易获得。常规凝胶电泳技术所需的许多实验室设备可以使用现成的组件以最简单的形式制造。为高中生实施了一个短期生物化学培训项目，使用新改进的设备为他们提供“实践”培训，这被证明是学习生化凝胶分离技术的一种令人兴奋的方式。© 2018国际生物化学与分子生物学联盟，46(5):566 - 577，2018。
