一维十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（1D SDS-PAGE）。

One-dimensional SDS-polyacrylamide gel electrophoresis (1D SDS-PAGE).

作者信息

Brunelle Julie L, Green Rachel

机构信息

Howard Hughes Medical Institute, Johns Hopkins University School of Medicine, Baltimore, MD, USA; Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD, USA.

出版信息

Methods Enzymol. 2014;541:151-9. doi: 10.1016/B978-0-12-420119-4.00012-4.

DOI:10.1016/B978-0-12-420119-4.00012-4

PMID:24674069

Abstract

This protocol describes a denaturing polyacrylamide gel system utilizing sodium dodecyl sulfate (SDS) to separate protein molecules based on size as first described by Laemmli (1970). SDS-PAGE can be used to monitor protein purifications, check the purity of samples, and to estimate molecular weights for unknown proteins.

摘要

本方案描述了一种变性聚丙烯酰胺凝胶系统，该系统利用十二烷基硫酸钠（SDS）根据大小分离蛋白质分子，这是Laemmli于1970年首次描述的。SDS-PAGE可用于监测蛋白质纯化过程、检查样品纯度以及估计未知蛋白质的分子量。