Nelson-Vasilchik Kimberly, Hague Joel, Mookkan Muruganantham, Zhang Zhanyuan J, Kausch Albert
Department of Cell and Molecular Biology, University of Rhode Island, Kingston, Rhode Island.
Plant Transformation Core Facility, Division of Plant Sciences, University of Missouri, Columbia, Missouri.
Curr Protoc Plant Biol. 2018 Dec;3(4):e20076. doi: 10.1002/cppb.20076. Epub 2018 Oct 17.
Most reliable transformation protocols for cereal crops, including sorghum (Sorghum bicolor L. Moench), rely on the use of immature embryo explants to generate embryogenic callus cells that are then transformed using Agrobacterium- or particle-bombardment-mediated DNA delivery. Subsequent to DNA transfer, most protocols rely on selectable markers for the recovery of stably transformed callus that is then regenerated to produce T plants. However, these protocols require specific genotypes that are innately capable of efficient embryogenic callus initiation. Here, we describe a system that makes use of the differential expression of the morphogenic regulators Baby Boom (Bbm) and Wuschel2 (Wus2) to achieve transformation in varieties of sorghum typically recalcitrant to standard transformation methods. © 2018 by John Wiley & Sons, Inc.
包括高粱（Sorghum bicolor L. Moench）在内的大多数可靠的谷类作物转化方案，都依赖于使用未成熟胚外植体来产生胚性愈伤组织细胞，然后使用农杆菌介导或粒子轰击介导的DNA传递方法对其进行转化。在DNA转移之后，大多数方案依赖于选择标记来筛选稳定转化的愈伤组织，然后将其再生以产生T代植株。然而，这些方案需要特定的基因型，这些基因型天生就能够高效启动胚性愈伤组织。在此，我们描述了一种利用形态发生调节因子婴儿潮（Baby Boom，Bbm）和Wuschel2（Wus2）的差异表达，在通常对标准转化方法具有抗性的高粱品种中实现转化的系统。© 2018约翰威立国际出版公司
