Li Jun, Yu Xiaoxiao, Zhang Chao, Li Na, Zhao Jianjun
State Key Laboratory of North China Crop Improvement and Regulation, College of Life Sciences, Hebei Agricultural University, Baoding, 071001 China.
Key Laboratory of Vegetable Germplasm Innovation and Utilization of Hebei, Collaborative Innovation Center of Vegetable Industry in Hebei, College of Horticulture, Hebei Agricultural University, Baoding, 071001 China.
aBIOTECH. 2022 Jul 2;3(2):146-161. doi: 10.1007/s42994-022-00076-3. eCollection 2022 Jun.
species are a global source of nutrients and edible vegetable oil for humans. However, all commercially important crops underwent a whole-genome triplication event, hindering the development of functional genomics and breeding programs. Fortunately, clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated (Cas) technologies, by allowing multiplex and precise genome engineering, have become valuable genome-editing tools and opened up new avenues for biotechnology. Here, we review current progress in the use of CRISPR/Cas technologies with an emphasis on the latest breakthroughs in precise genome editing. We also summarize the application of CRISPR/Cas technologies to crops for trait improvements. Finally, we discuss the challenges and future directions of these technologies for comprehensive application in crops. Ongoing advancement in CRISPR/Cas technologies, in combination with other achievements, will play a significant role in the genetic improvement and molecular breeding of crops.
物种是人类营养和食用植物油的全球来源。然而，所有具有商业重要性的作物都经历了一次全基因组三倍化事件，这阻碍了功能基因组学和育种计划的发展。幸运的是，成簇规律间隔短回文重复序列（CRISPR）/CRISPR相关蛋白（Cas）技术通过实现多重和精确的基因组工程，已成为有价值的基因组编辑工具，并为生物技术开辟了新途径。在这里，我们回顾了CRISPR/Cas技术使用的当前进展，重点是精确基因组编辑的最新突破。我们还总结了CRISPR/Cas技术在作物性状改良中的应用。最后，我们讨论了这些技术在作物全面应用中的挑战和未来方向。CRISPR/Cas技术的不断进步，与其他成果相结合，将在作物的遗传改良和分子育种中发挥重要作用。
