Yuan Pu, Usman Muhammad, Liu Wenshan, Adhikari Ashna, Zhang Chunquan, Njiti Victor, Xia Ye
Department of Plant Pathology, College of Food, Agricultural and Environmental Sciences, The Ohio State University, Columbus, Ohio, USA.
Department of Plant Pathology, University of Agriculture, Faisalabad, Pakistan.
Biotechnol J. 2024 Dec;19(12):e202400457. doi: 10.1002/biot.202400457.
Plant gene editing technology has significantly advanced in recent years, thereby transforming both biotechnological research and agricultural practices. This review provides a comprehensive summary of recent advancements in this rapidly evolving field, showcasing significant discoveries from improved transformation efficiency to advanced construct design. The primary focus is on the maturation of the Clustered regularly interspaced short palindromic repeats/CRISPR-associated protein (CRISPR/Cas)9 system, which has emerged as a powerful tool for precise gene editing in plants. Through a detailed exploration, we elucidate the intricacies of integrating genetic modifications into plant genomes, shedding light on transport mechanisms, transformation techniques, and optimization strategies specific to CRISPR constructs. Furthermore, we explore the initiatives aimed at extending the frontiers of gene editing to nonmodel plant species, showcasing the growing scope of this technology. Overall, this comprehensive review highlights the significant impact of recent advancements in plant gene editing, illuminating its transformative potential in driving agricultural innovation and biotechnological progress.
近年来，植物基因编辑技术取得了显著进展，从而改变了生物技术研究和农业实践。本综述全面总结了这一快速发展领域的最新进展，展示了从提高转化效率到先进构建体设计等方面的重大发现。主要重点是成簇规律间隔短回文重复序列/CRISPR相关蛋白9（CRISPR/Cas）系统的成熟，该系统已成为植物精确基因编辑的强大工具。通过详细探讨，我们阐明了将基因修饰整合到植物基因组中的复杂性，揭示了CRISPR构建体特有的转运机制、转化技术和优化策略。此外，我们探讨了旨在将基因编辑前沿扩展到非模式植物物种的举措，展示了这项技术不断扩大的应用范围。总体而言，这篇全面的综述突出了植物基因编辑最新进展的重大影响，阐明了其在推动农业创新和生物技术进步方面的变革潜力。
