Wang De Zhou, Mo Xiao Ting, Zhang Xia, Xu Miao Yun, Zhao Jun, Wang Lei
Biotechnology Research Institute, Chinese Academy of Agricultural Science, Beijing 100081, China.
Yi Chuan. 2018 Sep 20;40(9):767-778. doi: 10.16288/j.yczz.18-119.
Maize has become the most widely planted crops in China and improving maize stress tolerance is one of major target traits for maize breeding. C2H2 zinc finger proteins are widely involved in growth development and stress response in plants. In this study, the transcription factor ZmC2H2-1 gene was isolated from maize and its function was investigated. Our data showed that ZmC2H2-1 belonged to C2H2 transcription factor family, mainly located in the nucleus, and cannot self-activate in yeast. Drought, salt and ABA can inhibit ZmC2H2-1 expression in maize. The water loss rate of excised-leaves was faster in ZmC2H2-1-transgenic Arabidopsis than that in WT. When treated with PEG, high salt and ABA, the stress tolerance was more sensitive in ZmC2H2-1-transgenicplants than WT. These data showed that ZmC2H2-1 played a negative role in stress tolerance in maize. Collectively, this study provides important information for us to analyze ZmC2H2-1 regulatory network and mechanism of stress tolerance in maize.
玉米已成为中国种植最广泛的作物，提高玉米的抗逆性是玉米育种的主要目标性状之一。C2H2锌指蛋白广泛参与植物的生长发育和胁迫响应。本研究从玉米中分离出转录因子ZmC2H2-1基因并对其功能进行了研究。我们的数据表明，ZmC2H2-1属于C2H2转录因子家族，主要定位于细胞核，在酵母中不能自我激活。干旱、盐和脱落酸能抑制玉米中ZmC2H2-1的表达。ZmC2H2-1转基因拟南芥离体叶片的失水率比野生型快。用聚乙二醇、高盐和脱落酸处理时，ZmC2H2-1转基因植物的胁迫耐受性比野生型更敏感。这些数据表明，ZmC2H2-1在玉米的胁迫耐受性中起负作用。总的来说，本研究为我们分析ZmC2H2-1调控网络和玉米抗逆机制提供了重要信息。
