Nakagawa Kouichi, Minakawa Satoko, Itabashi Chieko, Sawamura Daisuke
Division of Regional Innovation, Graduate School of Health Sciences, Hirosaki University.
Department of Dermatology, Graduate School of Medicine, Hirosaki University.
Anal Sci. 2019 Mar 10;35(3):265-269. doi: 10.2116/analsci.18P367. Epub 2018 Oct 26.
Basal cell carcinoma (BCC) in paraffin-embedded specimens was investigated by nondestructively electron paramagnetic resonance (EPR) and X-band (9.45 GHz) EPR imaging (EPRI). A histopathological examination of specimens showed the melanin contents and revealed that they were predominantly of the nodular types. A single-line EPR pattern was observed in the BCC specimens, and the spectra of the samples were analyzed using linewidth and spectral pattern parameters. The eumelanin-related radical was observed in paraffin-embedded BCC specimens. The stability of the radical was supported by sepia pigment experiments. The g-value and peak-to-peak linewidths (ΔH) were 2.004 and 0.61 ± 0.02 mT (mean ± SEM), respectively. Strong EPRI signals correspond to areas of strong pigmentation in the samples. Thus, histopathological examination, EPR, and EPRI of BCC specimens showed that the radical distribution reflects on the spatial distribution of pigments in the samples.
采用非破坏性电子顺磁共振（EPR）和X波段（9.45 GHz）电子顺磁共振成像（EPRI）对石蜡包埋标本中的基底细胞癌（BCC）进行了研究。标本的组织病理学检查显示了黑色素含量，并表明它们主要为结节型。在BCC标本中观察到单线EPR模式，并使用线宽和光谱模式参数对样品的光谱进行了分析。在石蜡包埋的BCC标本中观察到了真黑素相关自由基。乌贼墨色素实验证实了该自由基的稳定性。g值和峰-峰线宽（ΔH）分别为2.004和0.61±0.02 mT（平均值±标准误）。强EPRI信号对应于样品中色素沉着强烈的区域。因此，BCC标本的组织病理学检查、EPR和EPRI表明，自由基分布反映了样品中色素的空间分布。
