Nakagawa Kouichi, Epel Boris
Division of Regional Innovation, Graduate School of Health Sciences, Hirosaki University.
J Oleo Sci. 2017 Mar 1;66(3):315-319. doi: 10.5650/jos.ess16152. Epub 2017 Feb 10.
This study investigated the location and distribution of paramagnetic species in apple seeds using electron paramagnetic resonance (EPR) and X-band (9 GHz) EPR imaging (EPRI). EPR primarily detected two paramagnetic species per measured seed. These two different radical species were assigned as stable radicals and Mn species based on the g values and hyperfine components. The signal from the stable radical was noted at g ≈ 2.00 and was strong and relatively stable. The subsequent noninvasive EPRI of the radical present in each seed revealed that the stable radicals were located primarily in the seed coat, with very few radicals observed in the cotyledon of the seed. These results indicate that the stable radical species were only found within the seed coat, and few radical species were found in other seed parts.
本研究利用电子顺磁共振（EPR）和X波段（9 GHz）电子顺磁共振成像（EPRI）研究了苹果种子中顺磁物质的位置和分布。EPR主要在每个被测种子中检测到两种顺磁物质。根据g值和超精细成分，这两种不同的自由基物种被确定为稳定自由基和锰物种。稳定自由基的信号在g≈2.00处被检测到，且信号强且相对稳定。随后对每个种子中存在的自由基进行的非侵入性EPRI显示，稳定自由基主要位于种皮中，在种子子叶中观察到的自由基很少。这些结果表明，稳定自由基物种仅存在于种皮内，在种子的其他部分很少发现自由基物种。
