Pedraza Luz Adriana, Bautista Jessica, Uribe-Vélez Daniel
Instituto de Biotecnología, Universidad Nacional de Colombia, A.A 14-490, Bogotá D.C., Colombia.
Plant Pathol J. 2018 Oct;34(5):393-402. doi: 10.5423/PPJ.OA.02.2018.0030. Epub 2018 Oct 1.
Rice world production is affected due to the growing impact of diseases such as bacterial panicle blight, produced by . The pathogen-induced symptoms include seedling rot, grain rot and leaf-sheath browning in rice plants. It is currently recognized the entrance of this pathogen to the plant, from infected seeds and from environmental sources of the microorganism. However, it is still not fully elucidated the dynamics and permanence of the pathogen in the plant, from its entry until the development of disease symptoms in seedlings or panicles. In this work it was evaluated the infection of rice plants, starting from inoculated seeds and substrates, and its subsequent monitoring after infection. Various organs of the plant during the vegetative stage and until the beginning of the reproductive stage, were evaluated. In both inoculation models, the bacteria was maintained in the plant as an endophyte between 1 × 10 and 1 × 10 cfu of .g of plant throughout the vegetative stage. An increase of bacterial population towards initiation of the panicle was observed, and in the maturity of the grain, an endophyte population was identified in the flag leaf at 1 × 10 cfu of .g fresh weight of rice plant, conducting towards the symptoms of bacterial panicle blight. The results found, suggest that in rice plants developed from infected seeds or from the substrate, can colonize seedlings, establishing and maintaining a bacterial population over time, using rice plants as habitat to survive endophyticly until formation of bacterial panicle blight symptoms.
由于诸如细菌性穗枯病等病害的影响日益增大，全球水稻产量受到影响。细菌性穗枯病由[病原菌名称未给出]引起。病原菌引发的症状包括水稻植株的幼苗腐烂、谷粒腐烂和叶鞘褐变。目前已知该病原菌可通过受感染种子以及微生物的环境源进入植株。然而，从病原菌进入植株直至在幼苗或穗部出现病害症状这一过程中，其在植株内的动态变化和存续情况仍未完全阐明。在本研究中，从接种种子和基质开始对水稻植株的感染情况进行了评估，并在感染后对其进行了后续监测。对营养生长阶段直至生殖生长阶段开始时植株的各个器官进行了评估。在两种接种模型中，在整个营养生长阶段，细菌在植株内以内生菌的形式维持在每克植株1×10至1×10[cfu数值未完整给出]cfu的[细菌名称未完整给出]水平。观察到在穗开始形成时细菌数量增加，在谷粒成熟时，在剑叶中鉴定出内生菌数量为每克水稻植株鲜重1×10[cfu数值未完整给出]cfu，这导致了细菌性穗枯病症状的出现。研究结果表明，从受感染种子或基质发育而来的水稻植株中的[细菌名称未完整给出]，能够定殖于幼苗，随着时间推移建立并维持细菌群体，以水稻植株为栖息地进行内生生存，直至细菌性穗枯病症状形成。
