Division of Biotechnology, Jeonbuk National University, Iksan-si, Jeollabuk-do, Republic of Korea.
Advanced Institute of Environment and Bioscience, Plant Medical Research Center, and Institute of Bio-industry, Jeonbuk National University, Jeonju-si, Jeollabuk-do, Republic of Korea.
PLoS One. 2023 Aug 14;18(8):e0290049. doi: 10.1371/journal.pone.0290049. eCollection 2023.
Burkholderia glumae causes bacterial panicle blight (BPB) and bacterial seedling rot (BSR) which are difficult to control in rice plants. Seed disinfection using microbes and eco-friendly materials is an efficient alternative practice for managing BPB and BSR. In this study, we applied Cytobacillus firmus JBRS159 (JBRS159) in combination with silicon dioxide (SiO2) nanoparticle or potassium silicate (K2SiO3) solution to control BSR. JBRS159, SiO2 nanoparticle, and K2SiO3 independently suppressed the BSR disease and promoted growths of rice and Arabidopsis. Population of B. glumae in the treated rice seeds was suppressed by the application of JBRS159 via competitions for nutrients and niches. The mixture of JBRS159 and each Si compound (SiO2 nanoparticle or K2SiO3) was complementary for disease-suppressing and growth-promoting activities of individual treatment. The results of this study indicate that mixture of JBRS159 with each Si compound can be harnessed for disease control and growth promotion as efficient alternatives to chemical pesticides and synthetic fertilizers. The efficacy of JBRS159 and Si compounds in the control of BSR and BPB in the field remains to be evaluated.
聚球藻伯克霍尔德氏菌引起的细菌性稻穗枯病（BPB）和细菌性苗腐病（BSR）是水稻生产上难以防治的病害。利用微生物和环保材料进行种子消毒是防治 BPB 和 BSR 的有效替代方法。在本研究中，我们将枯草芽孢杆菌 JBRS159（JBRS159）与二氧化硅（SiO2）纳米颗粒或硅酸钾（K2SiO3）溶液联合应用于防治 BSR。JBRS159、SiO2 纳米颗粒和 K2SiO3 均能单独抑制 BSR 病害并促进水稻和拟南芥的生长。通过与 JBRS159 的竞争，减少了水稻种子中聚球藻伯克霍尔德氏菌的种群数量，从而抑制了 BSR 的发生。JBRS159 与每种 Si 化合物（SiO2 纳米颗粒或 K2SiO3）的混合物在抑制病害和促进生长方面具有互补作用，优于单一处理。本研究结果表明，JBRS159 与每种 Si 化合物的混合物可作为防治病害和促进生长的有效替代物，替代化学农药和合成肥料。JBRS159 和 Si 化合物在田间防治 BSR 和 BPB 的效果仍有待评估。
