The Formation of the Goldfish-Like Fish Derived From Hybridization of Female Koi Carp × Male Blunt Snout Bream.

Goldfish ( var., GF; 2 = 100) is the most popular ornamental fish in the world. It is assumed that GF evolved from red crucian carp ( red var., RCC; 2 = 100). However, this hypothesis lacks direct evidence. Furthermore, our knowledge of the role of hybridization in the formation of new species is sparse. In this study, goldfish-like fish with twin tails (GF-L; 2 = 100) was produced by self-mating red crucian carp-like fish (RCC-L; 2 = 100) derived from the distant crossing of koi carp (, KOC; 2 = 100; ♀) with blunt snout bream (, BSB; 2 = 48; ♂). The phenotypes and genotypes of GF-L and RCC-L were very similar to those of GF and RCC, respectively. Microsatellite DNA and 5S rDNA analyses revealed that GF-L and RCC-L were closely related to GF and RCC, respectively. The presence of a twin tail of GF-L was related to a base mutation in from G in RCC-L to T in GF-L, indicating that the lineage of RCC-L and GF-L can be used to study gene variation and function. The sequences of 5S rDNA in GF-L and RCC-L were mapped to the genomes of CC and BSB, which revealed that the average similarities of both GF-L and RCC-L to CC were obviously higher than those to BSB, supporting that the genomes of both RCC-L and GF-L were mainly inherited from KOC. GF-L and RCC-L were homodiploids that were mainly derived from the genome of KOC with some DNA fragments from BSB. The reproductive traits of GF-L and RCC-L were quite different from those of their parents, but were the same as those of GF and RCC. RCC-L easily diversified into GF-L, suggesting that RCC and GF evolved within the same period in their evolutionary pathway. This study provided direct evidence of the KOC-RCC-GF evolutionary pathway that was triggered by distant hybridization, which had important significance in evolutionary biology and genetic breeding.