CAS Key Laboratory of Mountain Ecological Restoration and Bioresource Utilization & Ecological Restoration Biodiversity Conservation Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, 610041, People's Republic of China.
University of Chinese Academy of Sciences, 100039, Beijing, People's Republic of China.
Sci Rep. 2018 Oct 30;8(1):15996. doi: 10.1038/s41598-018-34449-w.
A complete randomized design was implemented with two watering regimes (extreme rainfall and control) and three different plant combinations (Zanthoxylum bungeanum, Z. bungeanum + Capsicum annum, Z. bungeanum + Glycine max) in order to assess the morphological and physio-biochemical responses of focal and neighbor plants. The results indicated that, extreme rainfall had significantly negative impacts on Z. bungeanum in three intercropping systems. However, intercropping with G. max improved the transpiration rate (T) and stomatal conductance (G), raised leaf relative water content (LRWC), increased chlorophyll a (Chl a) and carotenoid (Car) content, and enhanced the superoxide dismutase activity (SOD) of Z. bungeanum. After recovery, the Z. bungeanum + G. max mixed culture significantly increased soil NO-N, improved the intercellular carbon dioxide concentration (C) and T, upregulated soluble sugar and proline, and enhanced hydrogen peroxidase activity (CAT). Moreover, the higher root biomass of G. max provided much more nitrogen for Z. bungeanum via the return of organic matter. However, intercropping with C. annum significantly increased active oxygen (ROS). Compared with neighboring species, in intercropping systems, G. max could have improved the tolerance of the focal species Z. bungeanum in response to extreme rainfall and its recovery after extreme rainfall.
采用完全随机设计，设置极端降雨和对照 2 种水分处理，以及 3 种不同的植物组合（花椒、花椒+辣椒、花椒+大豆），以评估焦点植物和邻体植物的形态和生理生化响应。结果表明，极端降雨对 3 种间作系统中的花椒均产生显著的负面影响。然而，与大豆间作提高了花椒的蒸腾速率（T）和气孔导度（G），增加了叶片相对含水量（LRWC），提高了叶绿素 a（Chl a）和类胡萝卜素（Car）含量，并增强了花椒的超氧化物歧化酶活性（SOD）。恢复后，花椒+大豆混作显著增加了土壤硝态氮（NO-N），提高了胞间二氧化碳浓度（C）和 T，上调了可溶性糖和脯氨酸含量，并增强了过氧化氢酶活性（CAT）。此外，大豆较高的根生物量通过有机质的归还为花椒提供了更多的氮。然而，与辣椒间作显著增加了活性氧（ROS）。与邻体种相比，在间作系统中，大豆可能提高了焦点种花椒对极端降雨的耐受能力及其在极端降雨后的恢复能力。
