Yahara I, Kakimoto-Sameshima F
Proc Natl Acad Sci U S A. 1977 Oct;74(10):4511-5. doi: 10.1073/pnas.74.10.4511.
Most of the mobile receptors on mouse lymphocytes and thymocytes, including immunoglobulins, H-2 antigens, Thy-1.2 antigens, some concanavalin A receptors, and some antigenic determinants detected by anti-thymocyte serum, were redistributed into caps when the cells were incubated in hypertonic medium (about 600 mOsM) in the absence of ligands. The caps reverted to the original distributions if the cells were transferred again to isotonic medium. The viability of the cells was not decreased after incubation in the hypertonic medium. Ligand-independent cap formation appeared to depend upon cellular metabolism. Different species of receptors appeared to move with different mobilities during the process of ligand-independent cap formation. Most microvilli on cells showing caps in hypertonic medium were associated with the regions of the caps. These results suggest that free receptors can be induced to form caps if the receptors are allowed to interact with the machinery of cap formation under special conditions.
大多数小鼠淋巴细胞和胸腺细胞上的移动受体，包括免疫球蛋白、H-2抗原、Thy-1.2抗原、一些刀豆球蛋白A受体以及抗胸腺细胞血清检测到的一些抗原决定簇，在细胞于无配体的高渗培养基（约600毫渗量摩尔）中孵育时会重新分布形成帽状结构。如果将细胞再次转移到等渗培养基中，帽状结构会恢复到原来的分布状态。细胞在高渗培养基中孵育后活力并未降低。非配体依赖性帽状结构形成似乎依赖于细胞代谢。在非配体依赖性帽状结构形成过程中，不同种类的受体移动速度似乎不同。在高渗培养基中呈现帽状结构的细胞上的大多数微绒毛与帽状结构区域相关联。这些结果表明，如果在特殊条件下使受体与帽状结构形成机制相互作用，游离受体可被诱导形成帽状结构。
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