Yifrach Eden, Fischer Sven, Oeljeklaus Silke, Schuldiner Maya, Zalckvar Einat, Warscheid Bettina
Department of Molecular Genetics, Weizmann Institute of Science, 7610001, Rehovot, Israel.
Faculty of Biology and BIOSS Centre for Biological Signaling Studies, Biochemistry and Functional Proteomics, Institute for Biology II, University of Freiburg, 79104, Freiburg, Germany.
Subcell Biochem. 2018;89:47-66. doi: 10.1007/978-981-13-2233-4_2.
The current view on peroxisomes has changed dramatically from being human cell oddities to vital organelles that host several key metabolic pathways. To fulfil over 50 different enzymatic functions, human peroxisomes host either unique peroxisomal proteins or dual-localized proteins. The identification and characterization of the complete peroxisomal proteome in humans is important for diagnosis and treatment of patients with peroxisomal disorders as well as for uncovering novel peroxisomal functions and regulatory modules. Hence, here we compiled a comprehensive list of mammalian peroxisomal and peroxisome-associated proteins by curating results of several quantitative and non-quantitative proteomic studies together with entries in the UniProtKB and Compartments knowledge channel databases. Our analysis gives a holistic view on the mammalian peroxisomal proteome and brings to light potential new peroxisomal and peroxisome-associated proteins. We believe that this dataset, represents a valuable surrogate map of the human peroxisomal proteome.
目前对于过氧化物酶体的看法已发生了巨大变化，从被视为人类细胞中的奇特存在转变为承载多种关键代谢途径的重要细胞器。为了实现50多种不同的酶促功能，人类过氧化物酶体包含独特的过氧化物酶体蛋白或双定位蛋白。鉴定和表征人类完整的过氧化物酶体蛋白质组对于过氧化物酶体疾病患者的诊断和治疗以及揭示新的过氧化物酶体功能和调控模块至关重要。因此，我们通过整理多项定量和非定量蛋白质组学研究的结果以及UniProtKB和Compartments知识通道数据库中的条目，汇编了一份哺乳动物过氧化物酶体和过氧化物酶体相关蛋白的综合列表。我们的分析提供了对哺乳动物过氧化物酶体蛋白质组的整体看法，并揭示了潜在的新的过氧化物酶体和过氧化物酶体相关蛋白。我们相信，这个数据集代表了人类过氧化物酶体蛋白质组的有价值的替代图谱。
