Pan Ronghui, Hu Jianping
MSU-Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, MI, 48824, USA.
Plant Biology Department, Michigan State University, East Lansing, MI, 48824, USA.
Subcell Biochem. 2018;89:3-45. doi: 10.1007/978-981-13-2233-4_1.
Plant peroxisomes are required for a number of fundamental physiological processes, such as primary and secondary metabolism, development and stress response. Indexing the dynamic peroxisome proteome is prerequisite to fully understanding the importance of these organelles. Mass Spectrometry (MS)-based proteome analysis has allowed the identification of novel peroxisomal proteins and pathways in a relatively high-throughput fashion and significantly expanded the list of proteins and biochemical reactions in plant peroxisomes. In this chapter, we summarize the experimental proteomic studies performed in plants, compile a list of ~200 confirmed Arabidopsis peroxisomal proteins, and discuss the diverse plant peroxisome functions with an emphasis on the role of Arabidopsis MS-based proteomics in discovering new peroxisome functions. Many plant peroxisome proteins and biochemical pathways are specific to plants, substantiating the complexity, plasticity and uniqueness of plant peroxisomes. Mapping the full plant peroxisome proteome will provide a knowledge base for the improvement of crop production, quality and stress tolerance.
植物过氧化物酶体参与许多基本生理过程，如初级和次级代谢、发育及应激反应。对动态过氧化物酶体蛋白质组进行索引是全面了解这些细胞器重要性的先决条件。基于质谱（MS）的蛋白质组分析能够以相对高通量的方式鉴定新的过氧化物酶体蛋白和途径，并显著扩充了植物过氧化物酶体中的蛋白质和生化反应列表。在本章中，我们总结了在植物中进行的实验蛋白质组学研究，汇编了一份约200种已确认的拟南芥过氧化物酶体蛋白清单，并讨论了植物过氧化物酶体的多种功能，重点是基于拟南芥质谱的蛋白质组学在发现新的过氧化物酶体功能中的作用。许多植物过氧化物酶体蛋白和生化途径是植物特有的，这证实了植物过氧化物酶体的复杂性、可塑性和独特性。绘制完整的植物过氧化物酶体蛋白质组图谱将为提高作物产量、品质和抗逆性提供知识库。
