用二丁酰环磷腺苷、促卵泡素和人绒毛膜促性腺激素培养的牛卵母细胞的核成熟和胞质成熟。
Nuclear and cytoplasmic maturation of bovine oocytes cultured with dbc AMP, FSH and hCG.
作者信息
Liehman P, Greve T, Xu K P
出版信息
Acta Vet Scand. 1986;27(4):566-74. doi: 10.1186/BF03548136.
The present investigation was undertaken to study the effect of addition of dbc AMP on bovine oocyte maturation and fertilization in vitro. The bovine oocytes isolated from 2–8 mm follicles were cultured for 26 h in TCM-199. The maturation rate (71.4 %) did not significantly increase after supplementation of the culture medium with dbc AMP (86.3 %.) or FSH + hCG (86.3 %). The in vitro fertilization rate of oocytes based on sperm penetration and presence of sperm tail in the ooplasm increased significantly in the dbc AMP (34.7 %) and the dbc AMP + FSH + hCG (33.9 %) treated groups when compared with untreated controls (17.9 %). However, dbc AMP treated oocytes were not able to secure the formation of male pronucleus 20 h after in vitro fertilization, while in oocytes matured in dbc AMP free medium both pronuclei were present in approximately 15 % of the penetrated oocytes. Also, the sperm head decondensation was blocked or slowed down by the dbc AMP treatment. It is concluded (1) that dbc AMP may improve the condition for the interaction of oocytes with spermatozoa, and (2) that the ooplasm of such dbc AMP treated oocytes apparently is not able to decandense the sperm head and transform it to the male pronucleus.
本研究旨在探讨添加二丁酰环磷腺苷（dbc AMP）对牛卵母细胞体外成熟和受精的影响。从2 - 8毫米卵泡中分离出的牛卵母细胞在TCM - 199中培养26小时。在培养基中添加dbc AMP（86.3%）或促卵泡素（FSH）+人绒毛膜促性腺激素（hCG）（86.3%）后，成熟率（71.4%）没有显著提高。与未处理的对照组（17.9%）相比，在dbc AMP处理组（34.7%）和dbc AMP + FSH + hCG处理组（33.9%）中，基于精子穿透和卵质中精子尾部存在情况的卵母细胞体外受精率显著提高。然而，dbc AMP处理的卵母细胞在体外受精20小时后无法确保雄原核的形成，而在不含dbc AMP的培养基中成熟的卵母细胞中，约15%的穿透卵母细胞中存在两个原核。此外，dbc AMP处理会阻止或减缓精子头部的去浓缩。研究得出结论：（1）dbc AMP可能改善卵母细胞与精子相互作用的条件；（2）经dbc AMP处理的卵母细胞的卵质显然无法使精子头部去浓缩并将其转化为雄原核。
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