Böttcher M, Saedler J, Irmer P, Göbel D, Lange W
Sektion Tierproduktion, Universität Rostock.
Arch Exp Veterinarmed. 1991;45(1):81-5.
Studies were conducted into hormonal additives to medium and culturing time and their effects on in vitro maturation of bovine oocytes. The best maturation results were ensured in vitro by substitution of TCM-199 with FSH, HCG, and 17 beta-oestradiol: The stage of fertilisation capability (telophase I or metaphase II) was reached by 86% of all oocytes (115 of 134). The rate of maturation was worse with significance in FSH-free medium (62% or 101 of 163), and the amount of degenerated oocytes was twice as high (18%). Maturation in hormone-substituted medium for 28 to 30 hours is recommended under the condition that immature oocytes were cultured from juvenile antral follicles (2--5 mm in diameter).
对培养基中的激素添加剂和培养时间及其对牛卵母细胞体外成熟的影响进行了研究。通过用促卵泡素（FSH）、人绒毛膜促性腺激素（HCG）和17β-雌二醇替代TCM - 199，可在体外确保最佳成熟结果：所有卵母细胞（134个中的115个）中有86%达到受精能力阶段（第一次减数分裂末期或第二次减数分裂中期）。在无FSH培养基中，成熟率显著较低（163个中的62%或101个），退化卵母细胞数量高出一倍（18%）。在从未成熟卵泡（直径2 - 5毫米）培养未成熟卵母细胞的条件下，建议在激素替代培养基中培养28至30小时。
