Universidad de Buenos Aires (UBA), Facultad de Farmacia y Bioquímica, Departamento de Química Analítica y Fisicoquímica, (UBA), Junín 956, C1113AAD Buenos Aires, Argentina; Instituto de Química y Metabolismo del Fármaco, Fac. de Farmacia y Bioquímica, (IQUIMEFA-UBA-CONICET), Argentina.
Instituto de Investigaciones en Microbiología y Parasitología Médica (IMPaM), CONICET-Universidad de Buenos Aires, Paraguay 2155, C1121ABG piso 11, Buenos Aires, Argentina.
J Colloid Interface Sci. 2019 Feb 15;536:372-380. doi: 10.1016/j.jcis.2018.10.067. Epub 2018 Oct 24.
A smart antibacterial biomaterial based on a keratin hydrogel with pH-dependent behavior and Zinc Oxide nanoplates as biocide agent has been developed. The pH of a chronic wound is basic due to bacterial metabolism. Originally shrank at acid pH, keratin hydrogels swell upon contact with a bacterial contaminated media leading to the release of the nanoparticles. The material has been thoroughly characterized by infrared spectroscopy, Raman, scanning electron microscope, swelling behavior, Differential scanning calorimetry, Small-angle X-ray scattering, rheology, antimicrobial activity and cytotoxicity. The results show that 5% of Zinc Oxide nanoparticles concentration is the optimum for wound dressing applications.
已经开发出一种基于具有 pH 依赖性行为的角蛋白水凝胶和氧化锌纳米板作为杀菌剂的智能抗菌生物材料。由于细菌代谢，慢性伤口的 pH 值呈碱性。在酸性 pH 值下原本收缩的角蛋白水凝胶在接触到受细菌污染的介质时会膨胀，导致纳米颗粒释放。该材料通过红外光谱、拉曼光谱、扫描电子显微镜、溶胀行为、差示扫描量热法、小角 X 射线散射、流变学、抗菌活性和细胞毒性进行了全面表征。结果表明，氧化锌纳米粒子浓度为 5%时最适合用于伤口敷贴。
