Department of Chemistry, Bacha Khan University, Charsadda 24420, Khyber Pakhtunkhwa, Pakistan.
Department of Chemistry, Kohat University of Science and Technology, Kohat 26000, Khyber Pakhtunkhwa, Pakistan.
Molecules. 2023 Feb 27;28(5):2215. doi: 10.3390/molecules28052215.
Bacterial resistance to antibiotics and host defense systems is primarily due to bacterial biofilm formation in antibiotic therapy. In the present study, two complexes, bis (biphenyl acetate) bipyridine Cu (II) () and bis (biphenyl acetate) bipyridine Zn (II) (), were tested for their ability to prevent biofilm formation. The minimum inhibitory concentration and minimum bactericidal concentration of complexes and were 46.87 ± 1.822 and 93.75 ± 1.345 and 47.87 ± 1.345 and 94.85 ± 1.466 μg/mL, respectively. The significant activity of both complexes was attributed to the damage caused at the membrane level and was confirmed using an imaging technique. The biofilm inhibitory potential levels of complexes and were 95% and 71%, respectively, while the biofilm eradication potential levels were 95% and 35%, respectively, for both complexes. Both the complexes showed good interactions with the DNA. Thus, complexes and are good antibiofilm agents that exert their bactericidal actions possibly by disrupting the bacterial membrane and interacting with the bacterial DNA, which can act as a powerful agent to restrain the development of bacterial biofilm on therapeutic implants.
细菌对抗生素的耐药性和宿主防御系统主要是由于抗生素治疗中细菌生物膜的形成。在本研究中，测试了两个配合物，双（联苯乙酸）联吡啶 Cu（II）（）和双（联苯乙酸）联吡啶 Zn（II）（），以研究它们抑制生物膜形成的能力。配合物和的最小抑菌浓度和最小杀菌浓度分别为 46.87±1.822 和 93.75±1.345 μg/mL，以及 47.87±1.345 和 94.85±1.466 μg/mL。两种配合物的显著活性归因于在膜水平上造成的损伤，并通过成像技术得到证实。配合物和的生物膜抑制潜能水平分别为 95%和 71%，而生物膜清除潜能水平分别为 95%和 35%。两种配合物均与 DNA 表现出良好的相互作用。因此，配合物和是良好的抗生物膜剂，它们的杀菌作用可能是通过破坏细菌膜并与细菌 DNA 相互作用来发挥的，这可以作为一种强大的手段来抑制治疗植入物上细菌生物膜的发展。
