Moredun Research Institute, Pentlands Science Park, Bush Loan, Penicuik, Edinburgh, EH26 0PZ, Scotland, UK.
Institute of Immunology and Infection Research, The King's Buildings, Ashworth Laboratories, Charlotte Auerbach Road, Edinburgh, EH9 3FL, Scotland, UK.
Vet Res. 2018 Nov 1;49(1):112. doi: 10.1186/s13567-018-0608-9.
The presence of components of the RNA interference (RNAi) pathway in Psoroptes ovis, an ectoparasitic mite responsible for psoroptic mange, was investigated through interrogation of the P. ovis genome. Homologues of transcripts representing critical elements for achieving effective RNAi in the mite, Tetranychus urticae and the model organisms Caenorhabditis elegans and Drosophila melanogaster were identified and, following the development of a non-invasive immersion method of double stranded RNA delivery, gene silencing by RNAi was successfully demonstrated in P. ovis. Significant reductions in transcript levels were achieved for three target genes which encode the Group 2 allergen (Pso o 2), mu-class glutathione S-transferase (PoGST-mu1) and beta-tubulin (Poβtub). This is the first demonstration of RNAi in P. ovis and provides a mechanism for mining transcriptomic and genomic datasets for novel control targets against this economically important ectoparasite.
通过对 P. ovis 基因组的研究，调查了 RNA 干扰 (RNAi) 途径的成分在引起痒螨性皮炎的外寄生虫螨 Psoroptes ovis 中的存在情况。鉴定了与在螨 Tetranychus urticae 以及模式生物秀丽隐杆线虫和黑腹果蝇中实现有效 RNAi 的关键要素的转录本同源物，并在开发了非侵入性的双链 RNA 递送入侵方法后，成功地在 P. ovis 中实现了 RNAi 基因沉默。针对编码 Group 2 过敏原 (Pso o 2)、mu 类谷胱甘肽 S-转移酶 (PoGST-mu1) 和β-微管蛋白 (Poβtub) 的三个靶基因，成功实现了转录本水平的显著降低。这是在 P. ovis 中首次证明 RNAi 的存在，并为挖掘针对这种具有经济重要性的外寄生虫的新型控制靶标提供了一种转录组和基因组数据集的挖掘机制。
