Cabibi Daniela, Bisanti Andrea, Lanza Dorotea, Brucculeri Vanessa, Porcasi Rossana, Ingrao Sabrina, Midiri Federico, Picone Dario, Giannone Antonino Giulio
Departments of Sciences for the Promotion of Health and Mother and Child Care, Anatomic Pathology.
Radiological Sciences, University of Palermo, Palermo, Italy.
Appl Immunohistochem Mol Morphol. 2019 Jan;27(1):65-69. doi: 10.1097/PAI.0000000000000548.
Till date, the utility of cytologic assessment of ascites for the identification of hepatocellular carcinoma (HCC) cells is still debated and the usefulness of immunocytochemistry for glypican-3 (GPC3) and Hep Par-1 in this setting has not been reported. Liquid-based cytology of ascitic fluid of 28 cirrhotic patients was performed and the spots obtained were stained with hematoxylin and eosin, papanicolau, and with GPC3 and Hep Par-1 antibodies. GPC3 and Hep Par-1 antibodies stained positively the atypical cells in the ascites of 2 patients with HCC showing an exophytic growth pattern. The specimens of the patients with nonexophytic HCC, other non-HCC cancers, or cirrhosis stained negatively. We hypothesize a relationship with the exophytic growth of the tumor. Thus, GPC3 and Hep Par-1 are useful in the cytologic assessment of peritoneal effusions to distinguish mesothelial cells and neoplastic non-HCC cells from HCC cells, presence of which, however, represent a very rare event. This is the first study in which GPC3 and Hep Par-1 immunostaining has been used in the cytologic assessment of HCC ascites.
迄今为止，腹水的细胞学评估在识别肝细胞癌（HCC）细胞方面的效用仍存在争议，在这种情况下，免疫细胞化学检测磷脂酰肌醇蛋白聚糖-3（GPC3）和肝细胞石蜡1（Hep Par-1）的实用性尚未见报道。对28例肝硬化患者的腹水进行了液基细胞学检查，并将获取的涂片用苏木精-伊红、巴氏染色法染色，以及用GPC3和Hep Par-1抗体染色。GPC3和Hep Par-1抗体对2例呈外生性生长模式的HCC患者腹水中的非典型细胞呈阳性染色。非外生性HCC、其他非HCC癌症或肝硬化患者的标本染色均为阴性。我们推测这与肿瘤的外生性生长有关。因此，GPC3和Hep Par-1在腹水的细胞学评估中有助于区分间皮细胞、非HCC肿瘤细胞与HCC细胞，不过，HCC细胞的存在是非常罕见的情况。这是第一项将GPC3和Hep Par-1免疫染色用于HCC腹水细胞学评估的研究。
