Inoue Y, Hashimoto A, Takada Y, Nishimura K, Hiwada K, Kokubu T
Clin Exp Hypertens A. 1987;9(2-3):481-5. doi: 10.3109/10641968709164217.
Angiotensin converting enzyme (ACE) activities of bronchoalveolar lavage fluid (BALF) and serum in patients with sarcoidosis and with silicosis were measured. Serum ACE was measured by enzymic assay and radioimmunoassay. There was a close relationship between ACE activity and content (r = 0.78). Serum ACE activities in patients with active sarcoidosis (37.5 +/- 11.1 nmol/min/ml, mean +/- SD) and with silicosis (25.5 +/- 9.3) were significantly elevated from the control (18.6 +/- 6.0). BALF ACE activities in the control, patients with active sarcoidosis and with silicosis were 0.23 +/- 0.19 nmol/min/ml, 0.94 +/- 0.97 and 0.38 +/- 0.05, respectively. BALF ACE in patients with active sarcoidosis and with silicosis were significantly different from the control. When BALF ACE was corrected by the cell count of alveolar macrophage (per 10(6) cells), activity was significantly different from control only in the patients with sarcoidosis. Moreover, only the alveolar macrophages in sarcoidosis were stained by immunofluorescence and immunocytochemistry using rabbit antihuman ACE antibody. Induction of ACE in alveolar macrophage might have an important role for the activity or progression of sarcoidosis.
测定了结节病患者和矽肺患者支气管肺泡灌洗液（BALF）和血清中的血管紧张素转换酶（ACE）活性。血清ACE采用酶法和放射免疫法测定。ACE活性与含量之间存在密切关系（r = 0.78）。活动期结节病患者（37.5±11.1 nmol/min/ml，均值±标准差）和矽肺患者（25.5±9.3）的血清ACE活性较对照组（18.6±6.0）显著升高。对照组、活动期结节病患者和矽肺患者的BALF ACE活性分别为0.23±0.19 nmol/min/ml、0.94±0.97和0.38±0.05。活动期结节病患者和矽肺患者的BALF ACE与对照组有显著差异。当用肺泡巨噬细胞计数（每10⁶个细胞）校正BALF ACE时，只有结节病患者的活性与对照组有显著差异。此外，仅用兔抗人ACE抗体通过免疫荧光和免疫细胞化学方法对结节病患者的肺泡巨噬细胞进行了染色。肺泡巨噬细胞中ACE的诱导可能在结节病的活动或进展中起重要作用。
