Wickenden C, Malcolm A D, Coleman D V
Crit Rev Clin Lab Sci. 1987;25(1):1-18. doi: 10.3109/10408368709105875.
The increasing frequency of cervical neoplasia among younger women and the increased invasiveness of these tumors has led to a considerable growth in research into this disease. Conventional methods (epidemiology, cytology, and immunology), while being extremely useful, also have significant limitations. Recent advances in techniques for the manipulation of DNA now make it possible to analyze tissues for the presence of viral genomes. This review introduces these techniques and describes their application to the search for herpes simplex virus and human papillomavirus sequences in cervical tissue. The significance of the findings both for the mechanism of transmission of the disease, and also the consequences for early detection and hence more successful treatment, are also discussed.
年轻女性中宫颈肿瘤的发病率不断上升，且这些肿瘤的侵袭性增强，这使得对该疾病的研究大幅增加。传统方法（流行病学、细胞学和免疫学）虽然极为有用，但也有显著局限性。目前DNA操作技术的最新进展使得分析组织中病毒基因组的存在成为可能。本综述介绍了这些技术，并描述了它们在宫颈组织中寻找单纯疱疹病毒和人乳头瘤病毒序列方面的应用。还讨论了这些发现对于疾病传播机制的意义，以及对早期检测从而更成功治疗的影响。
