McDougall J K, Nelson J A, Myerson D, Beckmann A M, Galloway D A
J Invest Dermatol. 1984 Jul;83(1 Suppl):72s-76s. doi: 10.1111/1523-1747.ep12281204.
We are studying the role of sexually transmitted viruses in the development of human tumors. The persistence of herpes simplex virus, cytomegalovirus, and human papillomavirus nucleic acid sequences has been examined using cloned viral DNA sequences as probes. The relationship of the viruses to various stages in the progression of neoplasia is examined, with particular reference to the role of viral and/or cellular genes in the initiation, promotion, and maintenance of the neoplastic phenotype. The human tumors of major interest in this context are carcinomas of the cervix, vulva, and anus and Kaposi's sarcoma. The minimal fragment of HSV-2 DNA detected in cervical tumors is contained within a 656-bp sequence that can be used in transfection experiments to transform rodent cells in vitro to a malignant phenotype. However, neither this fragment nor any other is consistently retained in cervical tumors, suggesting that this viral DNA may initiate but not maintain the transformed phenotype.
我们正在研究性传播病毒在人类肿瘤发生过程中的作用。已使用克隆的病毒DNA序列作为探针，检测单纯疱疹病毒、巨细胞病毒和人乳头瘤病毒核酸序列的持续性。研究病毒与肿瘤形成各个阶段的关系，特别关注病毒和/或细胞基因在肿瘤表型的起始、促进和维持中的作用。在此背景下，主要关注的人类肿瘤是子宫颈癌、外阴癌、肛门癌和卡波西肉瘤。在子宫颈肿瘤中检测到的HSV - 2 DNA最小片段包含在一个656碱基对的序列中，该序列可用于转染实验，在体外将啮齿动物细胞转化为恶性表型。然而，这个片段或任何其他片段在子宫颈肿瘤中都不能持续保留，这表明这种病毒DNA可能启动但不能维持转化表型。
