Gunwant Pankaj, Pandey Anand Kumar, Kumar Aman, Singh Inderjeet, Kumar Sandeep, Phogat J B, Kumar Vinay, Patil C S, Tomar Piyush, Kumar Sunil, Magotra Ankit
Department of Veterinary Gynaecology and Obstetrics, Hisar, 125 004, Haryana, India.
Veterinary Clinical Complex, Hisar, 125 004, Haryana, India.
Anim Reprod Sci. 2018 Dec;199:51-59. doi: 10.1016/j.anireprosci.2018.10.006. Epub 2018 Oct 26.
The water buffalo have a seasonal pattern of reproduction with decreased sexual activity during the longer photoperiod. The present study was designed to identify a single nucleotide polymorphism (SNP) in the MTNR1 A gene and its association with seasonal reproduction and reproductive characteristics in Murrah buffalo cows. The 812 bp fragment encompassing exon II of the MTNR1 A gene was amplified from genomic DNA of 190 pluriparous Murrah buffalo cows. Amplified PCR products from 12 samples were subjected to custom sequencing of both ends (5' and 3' ends). A synonymous mutation was identified at position 72 in exon II of MTNR1 A gene. Digestion of PCR products with HpaI enzyme indicated there was one polymorphic site caused by the presence of nucleotide C at position 72 in place of T that resulted in three genotypes: T/T (812bp), C/T (812, 743 and 69 bp) and C/C (743 and 69 bp). There was an association (P < 0.05) between the MTNR1 A genotype and reproductive activity in Murrah buffalo cows such that buffalo cows with T/T genotype had less seasonal reproductive activity as compared with those with the C/C genotype. The period of greatest mating activity of buffalo cows with the C/C genotype was from November to December whereas buffalo cows with the T/T genotype mated mainly between May and July. Thus, the polymorphism of the MTNR1 A gene might be considered as a genetic marker to identify Murrah buffalo, which are able to reproduce during periods that are not a part of the typical breeding season for these buffalo.
水牛具有季节性繁殖模式，在较长光照周期内性活动会减少。本研究旨在鉴定MTNR1 A基因中的单核苷酸多态性（SNP）及其与穆拉水牛母牛季节性繁殖和繁殖特征的关联。从190头经产穆拉水牛母牛的基因组DNA中扩增出包含MTNR1 A基因外显子II的812 bp片段。对12个样本的扩增PCR产物进行两端（5'和3'端）的定制测序。在MTNR1 A基因外显子II的第72位鉴定出一个同义突变。用HpaI酶消化PCR产物表明，由于第72位核苷酸C取代了T，存在一个多态性位点，产生了三种基因型：T/T（812bp）、C/T（812、743和69 bp）和C/C（743和69 bp）。MTNR1 A基因型与穆拉水牛母牛的繁殖活动之间存在关联（P < 0.05），即与C/C基因型的水牛母牛相比，T/T基因型的水牛母牛季节性繁殖活动较少。C/C基因型的水牛母牛交配活动最频繁的时期是11月至12月，而T/T基因型的水牛母牛主要在5月至7月交配。因此，MTNR1 A基因的多态性可被视为一种遗传标记，用于鉴定那些能够在这些水牛典型繁殖季节之外的时期进行繁殖的穆拉水牛。
