Winter E, Krawinkel U, Radbruch A
EMBO J. 1987 Jun;6(6):1663-71. doi: 10.1002/j.1460-2075.1987.tb02415.x.
Immunoglobulin class switch recombination occurs at frequencies of up to 10%/cell/generation in activated murine B-lymphocytes. We analysed cH gene rearrangements and switch recombinations from active and inactive IgH loci of B-cells activated in various ways and immortalized by cell fusion. Although about half of the IgM+ cells show rearrangement of c mu genes, the deletion of c mu is a rare event. Half of the IgG3+ and IgG1+ cells show rearrangement of c mu genes on the inactive IgH locus and the other half of the IgG+ cells have deleted c mu from both IgH loci by switch recombination. This recombination is directed to the same switch regions on both IgH loci in 60-80% of all cases. Interleukin 4 may play a critical role in programming murine B-lymphocytes for specific switch recombination.
免疫球蛋白类别转换重组在活化的小鼠B淋巴细胞中发生的频率高达10%/细胞/代。我们分析了通过各种方式活化并经细胞融合永生化的B细胞的活性和非活性IgH基因座的重链基因重排和类别转换重组。虽然约一半的IgM+细胞显示μ链基因重排，但μ链的缺失是罕见事件。一半的IgG3+和IgG1+细胞在非活性IgH基因座上显示μ链基因重排，另一半IgG+细胞通过类别转换重组从两个IgH基因座上删除了μ链。在所有病例的60-80%中，这种重组指向两个IgH基因座上相同的转换区域。白细胞介素4可能在为小鼠B淋巴细胞进行特定的类别转换重组编程中起关键作用。
