BASF Agricultural Solutions Seed US LLC, 3500 Paramount Parkway, Morrisville, NC, 27560, USA.
ERI de Biotecnología y Biomedicina (BIOTECMED), Department of Genetics, Universitat de València, 46100, Burjassot, Spain.
Sci Rep. 2018 Nov 1;8(1):16204. doi: 10.1038/s41598-018-34214-z.
A modified Vip3C protein has been developed that has a spectrum of activity that has the potential to be commercially useful for pest control, and shows good efficacy against Spodoptera frugiperda in insect bioassays and field trials. For the first time Vip3A and Vip3C proteins have been compared to Cry1 and Cry2 proteins in a complete set of experiments from insect bioassays to competition binding assays to field trials, and the results of these complementary experiments are in agreement with each other. Binding assays with radiolabelled toxins and brush border membrane vesicles from S. frugiperda and Helicoverpa armigera show that the modified Vip3C protein shares binding sites with Vip3A, and does not share sites with Cry1F or Cry2A. In agreement with the resulting binding site model, Vip3A-resistant insects were also cross-resistant to the modified Vip3C protein. Furthermore, maize plants expressing the modified Vip3C protein, but not those expressing Cry1F protein, were protected against Cry1F-resistant S. frugiperda in field trials.
一种改良的 Vip3C 蛋白已经被开发出来，它具有潜在的商业用途，可以控制害虫，并且在昆虫生物测定和田间试验中对夜蛾表现出良好的效果。首次将 Vip3A 和 Vip3C 蛋白与 Cry1 和 Cry2 蛋白进行了全面比较，从昆虫生物测定到竞争结合测定再到田间试验，这些互补实验的结果相互一致。用放射性标记毒素和 S. frugiperda 和 H. armigera 的刷状缘膜泡进行的结合测定表明，改良的 Vip3C 蛋白与 Vip3A 共享结合位点，与 Cry1F 或 Cry2A 不共享结合位点。与所得的结合位点模型一致，Vip3A 抗性昆虫也对改良的 Vip3C 蛋白具有交叉抗性。此外，表达改良的 Vip3C 蛋白的玉米植株，而不是表达 Cry1F 蛋白的玉米植株，在田间试验中对 Cry1F 抗性的夜蛾具有保护作用。
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