一种用无水氟化氢从真菌和酵母中提取DNA的简单方法。

A simple method for extraction of DNA from fungi and yeasts with anhydrous hydrogen fluoride.

作者信息

Oshiro S, Katsura N, Kitada K, Gunge N

出版信息

FEBS Lett. 1987 Aug 17;220(2):383-6. doi: 10.1016/0014-5793(87)80851-7.

Abstract

A novel method is described for the extraction of DNAs from fungi and yeasts. Anhydrous hydrogen fluoride (HF) selectively cleaves their cell walls under mild conditions (for 5 min at 0 degrees C), enabling the effective extraction of DNAs from organisms with a cell wall. A possible mechanism for this method concerning the selective cleavage of O-glycosidic linkages in cell walls has been described previously [(1977) Anal. Biochem. 82,289-309]. The extracted DNA is intact: in fact, the yeast DNA is directly applicable for restriction analysis and transformation of Escherichia coli.

摘要

描述了一种从真菌和酵母中提取DNA的新方法。无水氟化氢（HF）在温和条件下（0℃下5分钟）能选择性地裂解它们的细胞壁，从而能够从具有细胞壁的生物体中有效提取DNA。先前已描述了该方法关于细胞壁中O-糖苷键选择性裂解的一种可能机制[（1977年）《分析生物化学》82卷，289 - 309页]。提取的DNA是完整的：实际上，酵母DNA可直接用于限制性分析和大肠杆菌的转化。