一种通过聚合酶链反应筛选大量酵母克隆的快速可靠的DNA制备方法。 - Suppr

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超能文献

A rapid and reliable DNA preparation method for screening a large number of yeast clones by polymerase chain reaction.

作者信息

Ling M, Merante F, Robinson B H

机构信息

Department of Genetics, Hospital for Sick Children, Toronto, Ontario, Canada.

出版信息

Nucleic Acids Res. 1995 Dec 11;23(23):4924-5. doi: 10.1093/nar/23.23.4924.

DOI:10.1093/nar/23.23.4924

PMID:8532539

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC307485/

Abstract

摘要

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本文引用的文献

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Biotechniques. 1994 Sep;17(3):498-501.

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Gene. 1986;42(2):169-73. doi: 10.1016/0378-1119(86)90293-3.

Direct clone characterization from plaques and colonies by the polymerase chain reaction.

Nucleic Acids Res. 1989 May 25;17(10):4000. doi: 10.1093/nar/17.10.4000.

Systematic screening of yeast artificial-chromosome libraries by use of the polymerase chain reaction.

Proc Natl Acad Sci U S A. 1990 Feb;87(3):1213-7. doi: 10.1073/pnas.87.3.1213.

Recovery of plasmids from yeast into Escherichia coli: shuttle vectors.

Methods Enzymol. 1991;194:319-29. doi: 10.1016/0076-6879(91)94024-7.

Rapid analysis of yeast transformants using colony-PCR.

Biotechniques. 1992 Sep;13(3):350.

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