Shibata Y, Abiko Y, Arii H, Sone M, Takiguchi H
Int J Biochem. 1987;19(6):489-93. doi: 10.1016/0020-711x(87)90131-5.
This report describes a simple and efficient procedure for the isolation of plasma membrane from guinea pig peritoneal macrophages. The use of polycationic beads (Affi-gel 731 beads) facilitates rapid and high-clear separation of plasma membrane within 30 min. The final plasma membrane coated beads fraction has high specific activities of marker enzymes with little contamination with mitochondrial, lysosomal or cytoplasmal markers.
本报告描述了一种从豚鼠腹膜巨噬细胞中分离质膜的简单高效方法。使用聚阳离子珠（Affi-gel 731珠）有助于在30分钟内快速且高纯度地分离质膜。最终的质膜包被珠级分具有标记酶的高比活性，且几乎没有线粒体、溶酶体或细胞质标记物的污染。
