Reeves C D, Okita T W
Gene. 1987;52(2-3):257-66. doi: 10.1016/0378-1119(87)90052-7.
The alpha/beta-gliadin genes isolated from both hexaploid wheat (cv. Yamhill) and the diploid A genome progenitor Triticum urartu had remarkably similar sequences and differ by only a few point mutations. Primer extension analysis indicated that the transcriptional start points for individual genes in the family cluster within a few nucleotides. Comparison of the promoter region of several alpha/beta-gliadin and B-hordein genes reveals two conserved regions at about -130 and -250 bp. DNA from the hexaploid cultivars, Cheyenne and Chinese Spring, and the diploid progenitors T. urartu and Aegilops squarrosa was analysed by Southern blotting. Restriction fragment lengths of the alpha/beta-gliadin genes varied only slightly between the various wheats, although the overall copy number varied significantly. A region between approx. -1700 and -700 bp upstream from the TATA box was highly repeated in all three wheat genomes. For the hexaploid-derived gene, over 1700 bp of sequence upstream from the TATA box was determined, revealing an additional open reading frame between approx. -1550 and -1250 bp relative to the gliadin TATA box. Northern blot analysis indicated that RNA homologous to this repeated sequence family was present only in developing seed and accumulated to a maximum at late stages of maturation.
从六倍体小麦（品种Yamhill）和二倍体A基因组祖先乌拉尔图小麦中分离出的α/β-醇溶蛋白基因序列非常相似，仅存在少数点突变。引物延伸分析表明，该基因家族中各个基因的转录起始点集中在几个核苷酸范围内。对几个α/β-醇溶蛋白基因和B-大麦醇溶蛋白基因的启动子区域进行比较，发现了两个位于约-130和-250 bp处的保守区域。通过Southern杂交分析了六倍体品种Cheyenne和中国春以及二倍体祖先乌拉尔图小麦和节节麦的DNA。尽管α/β-醇溶蛋白基因的总体拷贝数差异很大，但不同小麦之间其限制性片段长度仅略有不同。在TATA框上游约-1700至-700 bp的区域在所有三个小麦基因组中都高度重复。对于源自六倍体的基因，确定了TATA框上游超过1700 bp的序列，发现在相对于醇溶蛋白TATA框约-1550至-1250 bp之间存在一个额外的开放阅读框。Northern杂交分析表明，与该重复序列家族同源的RNA仅存在于发育中的种子中，并在成熟后期积累到最大值。
